Retrieval of DNA using soft-landing after mass analysis by ESI-FTICR for enzymatic manipulation
The DNA modifications that lead to mutations are of direct interest for, e.g. the understanding of DNA damage recognition and repair. The characterization of the modified oligonucleotides and enzymatic responses to specific DNA modifications are of primary concern, but also present major analytical challenges. Among the available techniques, mass spectrometry has become an increasingly important tool for the study of oligonucleotides, their mutations, and interactions. Conventionally, mass spectrometry provides mass and structural information (e.g. from dissociation experiments and the use of tandem mass spectrometry). However, the small quantities of material analyzed and the destructive nature of conventional mass spectrometric detection (e.g., due to high energy impact on particle multiplier surfaces) have precluded subsequent use of mass separated biopolymers. The authors report the use of mass spectrometry in conjunction with soft-landing for the high-resolution analysis, separation, and selective collection of oligonucleotides, and their subsequent retrieval for enzymatic manipulation. Fourier transform ion cyclotron resonance mass spectrometry coupled with electrospray ionization (ESI-FTICR) allows nondestructive (i.e., image current) measurement of the mass-to-charge ratios (m/z) of ions with high sensitivity, resolution, and mass accuracy.
- Research Organization:
- Pacific Northwest National Lab., Richland, WA (US)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC06-76RL01830
- OSTI ID:
- 20000049
- Journal Information:
- Journal of the American Chemical Society, Vol. 121, Issue 38; Other Information: PBD: 29 Sep 1999; ISSN 0002-7863
- Country of Publication:
- United States
- Language:
- English
Similar Records
Characterization of PCR products from bacilli using electrospray ionization FTICR mass spectrometry
The study of large biopolymer complexes in solution and the gas phase using electrospray ionization-FTICR mass spectrometry