Lectin Receptor-like Kinase Signaling during Engineered Ectomycorrhiza Colonization

Shrestha, Him K.; Yao, Tao; Qiao, Zhenzhen; Muchero, Wellington; Hettich, Robert (Bob) L.; Chen, Jin-Gui (Jay); Abraham, Paul E.

doi:10.3390/cells12071082

Title: Lectin Receptor-like Kinase Signaling during Engineered Ectomycorrhiza Colonization

Journal Article · Tue Apr 04 00:00:00 EDT 2023 · Cells

DOI:https://doi.org/10.3390/cells12071082· OSTI ID:1973326

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Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Mutualistic association can improve a plant’s health and productivity. G-type lectin receptor-like kinase (PtLecRLK1) is a susceptibility factor in Populus trichocarpa that permits root colonization by a beneficial fungus, Laccaria bicolor. Engineering PtLecRLK1 also permits L. bicolor root colonization in non-host plants similar to Populus trichocarpa. The intracellular signaling reprogramed by PtLecRLK1 upon recognition of L. bicolor to allow for the development and maintenance of symbiosis is yet to be determined. In this study, phosphoproteomics was utilized to identify phosphorylation-based relevant signaling pathways associated with PtLecRLK1 recognition of L. bicolor in transgenic switchgrass roots. Our finding shows that PtLecRLK1 in transgenic plants modifies the chitin-triggered plant defense and MAPK signaling along with a significant adjustment in phytohormone signaling, ROS balance, endocytosis, cytoskeleton movement, and proteasomal degradation in order to facilitate the establishment and maintenance of L. bicolor colonization. Moreover, protein–protein interaction data implicate a cGMP-dependent protein kinase as a potential substrate of PtLecRLK1.

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Research Organization:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1973326

Journal Information:: Cells, Vol. 12, Issue 7; ISSN 2073-4409

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
phosphoproteomics
lectin receptor-like kinase
plant–microbe interaction
symbiosis
ectomycorrhiza colonization

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