Multilayered Ordered Protein Arrays Self-Assembled from a Mixed Population of Virus-like Particles

Uchida, Masaki; Brunk, Nicholas E.; Hewagama, Nathasha D.; Lee, Byeongdu; Prevelige, Peter E.; Jadhao, Vikram; Douglas, Trevor

doi:10.1021/acsnano.1c11272

Title: Multilayered Ordered Protein Arrays Self-Assembled from a Mixed Population of Virus-like Particles

Journal Article · Thu May 12 00:00:00 EDT 2022 · ACS Nano

DOI:https://doi.org/10.1021/acsnano.1c11272· OSTI ID:1969739

^[1];

^[2]; Hewagama, Nathasha D. ^[3];

^[4]; Prevelige, Peter E. ^[5];

^[3];

^[3]

California State University, Fresno, CA (United States); Indiana Univ., Bloomington, IN (United States)
Indiana Univ., Bloomington, IN (United States); Wolfram Research, Champaign, IL (United States); VeriSIM Life Inc., San Francisco, CA (United States)
Indiana Univ., Bloomington, IN (United States)
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Univ. of Alabama, Birmingham, AL (United States)

Biology shows many examples of spatially controlled assembly of cells and biomacromolecules into hierarchically organized structures, to which many of the complex biological functions are attributed. While such biological structures have inspired the design of synthetic materials, it is still a great challenge to control the spatial arrangement of individual building blocks when assembling multiple types of components into bulk materials. Here, in this study, we report self-assembly of multilayered, ordered protein arrays from mixed populations of virus-like particles (VLPs). We systematically tuned the magnitude of the surface charge of the VLPs via mutagenesis to prepare four different types of VLPs for mixing. A mixture of up to four types of VLPs selectively assembled into higher-order structures in the presence of oppositely charged dendrimers during a gradual lowering of the ionic strength of the solution. The assembly resulted in the formation of three-dimensional ordered VLP arrays with up to four distinct layers including a central core, with each layer comprising a single type of VLP. A coarse-grained computational model was developed and simulated using molecular dynamics to probe the formation of the multilayered, core-shell structure. Our findings establish a simple and versatile bottom-up strategy to synthesize multilayered, ordered materials by controlling the spatial arrangement of multiple types of nanoscale building blocks in a one-pot fabrication.

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Research Organization:: Argonne National Lab. (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC); National Science Foundation (NSF); National Institutes of Health (NIH)

Grant/Contract Number:: AC02-06CH11357; CMMI-1922883; DMR-1753182; 1720625; NIH1-S10OD024988-01

OSTI ID:: 1969739

Journal Information:: ACS Nano, Vol. 16, Issue 5; ISSN 1936-0851

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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Title: Multilayered Ordered Protein Arrays Self-Assembled from a Mixed Population of Virus-like Particles

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