Isolation, structure elucidation, and immunostimulatory activity of polysaccharide fractions from Boswellia carterii frankincense resin

Hosain, Najlaa A.; Ghosh, Rajarshi; Bryant, Daniel L.; Arivett, Brock A.; Farone, Anthony L.; Kline, Paul C.

doi:10.1016/j.ijbiomac.2019.04.059

Title: Isolation, structure elucidation, and immunostimulatory activity of polysaccharide fractions from Boswellia carterii frankincense resin

Journal Article · Thu Apr 11 00:00:00 EDT 2019 · International Journal of Biological Macromolecules

DOI:https://doi.org/10.1016/j.ijbiomac.2019.04.059· OSTI ID:1964091

Hosain, Najlaa A. ^[1]; Ghosh, Rajarshi ^[1]; Bryant, Daniel L. ^[1]; Arivett, Brock A. ^[1]; Farone, Anthony L. ^[1]; Kline, Paul C. ^[1]

Middle Tennessee State Univ., Murfreesboro, TN (United States)

Frankincense has a long history in religious, cultural, and medicinal use. In this study polysaccharides were extracted from frankincense from Boswellia carterii. The polysaccharides were purified by anion exchange chromatography on a DEAE-Sepharose Fast Flow 16/10 FPLC column. Six fractions were obtained and the three most active immunomodulatory fractions were further purified by size exclusion chromatography on a Superdex-200 column. The composition showed the monosaccharides present were predominantly galactose, arabinose, and glucuronic acid along with small amounts of rhamnose and glucose. The monosaccharide composition and glycosyl linkage analysis revealed the polysaccharides belong to the type II arabinogalactans. Fourier-transform infrared spectroscopy and bicinchoninic acid assay showed that the amount of protein in the samples was <1 wt%. One-dimensional ¹H NMR were consistent with high molecular weight compounds. The monosaccharides were primarily in the β conformation. The three fractions exhibited an immunostimulatory effect on RAW 264.7 murine macrophage cells. The most active immunostimulatory fraction FA2, stimulated a range of pro-inflammatory mediators including iNOS, NO, TNF-α, and IL-6 in RAW 264.7 cells. The fractions were effective in proliferating primary murine splenocytes. The results indicate that the polysaccharides isolated from frankincense have the potential to be used as an immunological stimulant or nutraceutical.

View Accepted Manuscript (DOE)

View Accepted Manuscript (Publisher)

Cite

Export

Save

Research Organization:: Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division; USDOE

Grant/Contract Number:: SC0015662

OSTI ID:: 1964091

Alternate ID(s):: OSTI ID: 1507810

Journal Information:: International Journal of Biological Macromolecules, Vol. 133; ISSN 0141-8130

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 13 works

Citation information provided by
Web of Science

References (36)

Frankincense oil derived from Boswellia carteri induces tumor cell specific cytotoxicity Frank, Mark Barton; Yang, Qing; Osban, Jeanette BMC Complementary and Alternative Medicine, Vol. 9, Issue 1 https://doi.org/10.1186/1472-6882-9-6	journal	March 2009
Phytochemistry and potential therapeutic actions of Boswellic acids: A mini-review Iram, Farah; Khan, Shah Alam; Husain, Asif Asian Pacific Journal of Tropical Biomedicine, Vol. 7, Issue 6 https://doi.org/10.1016/j.apjtb.2017.05.001	journal	June 2017
The effect of tapping for frankincense on sexual reproduction in Boswellia papyrifera Rijkers, Toon; Ogbazghi, Woldeselassie; Wessel, Marius Journal of Applied Ecology, Vol. 43, Issue 6 https://doi.org/10.1111/j.1365-2664.2006.01215.x	journal	August 2006
Composition and potential anticancer activities of essential oils obtained from myrrh and frankincense Chen, Yingli; Zhou, Chunlan; Ge, Zhendan Oncology Letters, Vol. 6, Issue 4 https://doi.org/10.3892/ol.2013.1520	journal	August 2013
Boswellia Basch, Ethan; Boon, Heather; Heerema, Theresa Davies Journal of Herbal Pharmacotherapy, Vol. 4, Issue 3 https://doi.org/10.1080/J157v04n03_06	journal	January 2004
Inhibition of Leukotriene B₄Formation in Rat Peritoneal Neutrophils by an Ethanolic Extract of the Gum Resin Exudate ofBoswellia serrata Ammon, H. P. T.; Mack, T.; Singh, G. Planta Medica, Vol. 57, Issue 03 https://doi.org/10.1055/s-2006-960074	journal	June 1991
The Structure of Frankincense Gum Jones, J. K. N.; Nunn, J. R. Journal of the American Chemical Society, Vol. 77, Issue 21 https://doi.org/10.1021/ja01626a089	journal	November 1955
770. The gum component of olibanum El-Khadem, H.; Megahed, M. M. Journal of the Chemical Society (Resumed) https://doi.org/10.1039/jr9560003953	journal	January 1956
Isolation and structure of a 4-O-methyl-glucuronoarabino-galactan from Boswellia serrata Sen, Ashis K.; Das, Asit K.; Banerji, Nilima Carbohydrate Research, Vol. 223 https://doi.org/10.1016/0008-6215(92)80031-U	journal	January 1992
Proteoglycans from Boswellia serrata Roxb. and B. carteri Birdw. and identification of a proteolytic plant basic secretory protein Herrmann, Andreas; König, Simone; Lechtenberg, Matthias Glycobiology, Vol. 22, Issue 11 https://doi.org/10.1093/glycob/cws107	journal	July 2012
Polysaccharides Isolated from Açaí Fruit Induce Innate Immune Responses Holderness, Jeff; Schepetkin, Igor A.; Freedman, Brett PLoS ONE, Vol. 6, Issue 2 https://doi.org/10.1371/journal.pone.0017301	journal	February 2011
Anticancer polysaccharides from natural resources: A review of recent research Zong, Aizhen; Cao, Hongzhi; Wang, Fengshan Carbohydrate Polymers, Vol. 90, Issue 4 https://doi.org/10.1016/j.carbpol.2012.07.026	journal	November 2012
Protective Effect of Ginseng Polysaccharides on Influenza Viral Infection Yoo, Dae-Goon; Kim, Min-Chul; Park, Min-Kyung PLoS ONE, Vol. 7, Issue 3 https://doi.org/10.1371/journal.pone.0033678	journal	March 2012
Botanical polysaccharides: Macrophage immunomodulation and therapeutic potential Schepetkin, Igor A.; Quinn, Mark T. International Immunopharmacology, Vol. 6, Issue 3 https://doi.org/10.1016/j.intimp.2005.10.005	journal	March 2006
A new vaccine adjuvant (BOS 2000) a potent enhancer mixed Th1/Th2 immune responses in mice immunized with HBsAg Khajuria, Anamika; Gupta, Amit; Malik, Fayaz Vaccine, Vol. 25, Issue 23 https://doi.org/10.1016/j.vaccine.2007.03.051	journal	June 2007
Immunomodulatory activity of biopolymeric fraction BOS 2000 fromBoswellia serrata Khajuria, Anamika; Gupta, Amit; Suden, Pankaj Phytotherapy Research, Vol. 22, Issue 3 https://doi.org/10.1002/ptr.2320	journal	January 2008
Immunological adjuvant effect of Boswellia serrata (BOS 2000) on specific antibody and cellular response to ovalbumin in mice Gupta, Amit; Khajuria, A.; Singh, J. International Immunopharmacology, Vol. 11, Issue 8 https://doi.org/10.1016/j.intimp.2011.02.011	journal	August 2011
The Isolation of the Components of Streptococcal Nucleoproteins in Serologically Active form Sevag, M. G.; Lackman, David B.; Smolens, J. Journal of Biological Chemistry, Vol. 124, Issue 2 https://doi.org/10.1016/S0021-9258(18)74048-9	journal	July 1938
Colorimetric Method for Determination of Sugars and Related Substances DuBois, Michel.; Gilles, K. A.; Hamilton, J. K. Analytical Chemistry, Vol. 28, Issue 3, p. 350-356 https://doi.org/10.1021/ac60111a017	journal	March 1956
Analysis of the Monosaccharide Components in Angelica Polysaccharides by High Performance Liquid Chromatography Yang, Xingbin; Zhao, Yan; Wang, Qingwei Analytical Sciences, Vol. 21, Issue 10 https://doi.org/10.2116/analsci.21.1177	journal	January 2005
Measurement of protein using bicinchoninic acid Smith, P. K.; Krohn, R. I.; Hermanson, G. T. Analytical Biochemistry, Vol. 150, Issue 1 https://doi.org/10.1016/0003-2697(85)90442-7	journal	October 1985
Suppression of LPS-induced NF-κB activity in macrophages by the synthetic aurone, (Z)-2-((5-(hydroxymethyl) furan-2-yl) methylene) benzofuran-3(2H)-one Park, Hyo S.; Nelson, David E.; Taylor, Zachary E. International Immunopharmacology, Vol. 43 https://doi.org/10.1016/j.intimp.2016.12.004	journal	February 2017
Preparation, preliminary characterization and immunostimulatory activity of polysaccharide fractions from the peduncles of Hovenia dulcis Wang, Mingchun; Jiang, Changxing; Ma, Liping Food Chemistry, Vol. 138, Issue 1 https://doi.org/10.1016/j.foodchem.2012.09.098	journal	May 2013
Extraction optimization, characterization and immunity activity of polysaccharides from Fructus Jujubae Cui, Guoting; Zhang, Wuxia; Wang, Qingjie Carbohydrate Polymers, Vol. 111 https://doi.org/10.1016/j.carbpol.2014.04.041	journal	October 2014
Frankincense – therapeutic properties Al-Yasiry, Ali Ridha Mustafa; Kiczorowska, Bożena Postępy Higieny i Medycyny Doświadczalnej, Vol. 70 https://doi.org/10.5604/17322693.1200553	journal	January 2016
Comparison of Chemical Analyses of Hollow-Fiber Dialyzer Extracts Pearson, Frederick C.; Bohon, Joyce; Lee, Wanda Artificial Organs, Vol. 8, Issue 3 https://doi.org/10.1111/j.1525-1594.1984.tb04293.x	journal	August 1984
Gelation of Limulus Amoebocyte Lysate by Simple Polysaccharides Mikami, Takeshi; Nagase, Toshihiko; Matsumoto, Tatsuji Microbiology and Immunology, Vol. 26, Issue 5 https://doi.org/10.1111/j.1348-0421.1982.tb00190.x	journal	May 1982
Recent advances in Cordyceps sinensis polysaccharides: Mycelial fermentation, isolation, structure, and bioactivities: A review Yan, Jing-Kun; Wang, Wen-Qiang; Wu, Jian-Yong Journal of Functional Foods, Vol. 6 https://doi.org/10.1016/j.jff.2013.11.024	journal	January 2014
Form and function of arabinogalactans and arabinogalactan-proteins Clarke, A. E.; Anderson, R. L.; Stone, B. A. Phytochemistry, Vol. 18, Issue 4 https://doi.org/10.1016/S0031-9422(00)84255-7	journal	January 1979
Arabinogalactan-Proteins: Key Regulators at the Cell Surface? Ellis, Miriam; Egelund, Jack; Schultz, Carolyn J. Plant Physiology, Vol. 153, Issue 2 https://doi.org/10.1104/pp.110.156000	journal	April 2010
Nitric oxide and the immune response Bogdan, Christian Nature Immunology, Vol. 2, Issue 10 https://doi.org/10.1038/ni1001-907	journal	October 2001
Glucan-immunostimulant, adjuvant, potential drug Vetvicka, Vaclav World Journal of Clinical Oncology, Vol. 2, Issue 2 https://doi.org/10.5306/wjco.v2.i2.115	journal	January 2011
Innate immunity: an overview Beutler, B. Molecular Immunology, Vol. 40, Issue 12 https://doi.org/10.1016/j.molimm.2003.10.005	journal	February 2004
Macrophage Activation by the Polysaccharide Arabinogalactan Isolated From Plant Cell Cultures of Echinacea purpurea Luettig, B.; Steinmuller, C.; Gifford, G. E. JNCI Journal of the National Cancer Institute, Vol. 81, Issue 9 https://doi.org/10.1093/jnci/81.9.669	journal	May 1989
Relationship between structure and immunological activity of an arabinogalactan from Lycium ruthenicum Peng, Qiang; Liu, Hang; Lei, Hongjie Food Chemistry, Vol. 194 https://doi.org/10.1016/j.foodchem.2015.08.087	journal	March 2016
Carbohydrates analysis in herbal glycomics Li, Shao-ping; Wu, Ding-tao; Lv, Guang-ping TrAC Trends in Analytical Chemistry, Vol. 52 https://doi.org/10.1016/j.trac.2013.05.020	journal	December 2013

Similar Records

An acidic polysaccharide (AGC3) isolated from North American ginseng (Panax quinquefolius) suspension culture as a potential immunomodulatory nutraceutical

Journal Article · Wed Aug 05 00:00:00 EDT 2020 · Current Research in Food Science · OSTI ID:1964091

Ghosh, Rajarshi; Bryant, Daniel L.; Arivett, Brock A.; +4 more

Panax quinquefolius (North American ginseng) cell suspension culture as a source of bioactive polysaccharides: Immunostimulatory activity and characterization of a neutral polysaccharide AGC1

Journal Article · Wed Jul 31 00:00:00 EDT 2019 · International Journal of Biological Macromolecules · OSTI ID:1964091

Ghosh, Rajarshi; Smith, Shannon A.; Nwangwa, Evidence E.; +10 more

Immunomodulatory effects of polysaccharide fraction isolated from Fagopyrum esculentum on innate immune system

Journal Article · Thu Feb 15 00:00:00 EST 2018 · Biochemical and Biophysical Research Communications · OSTI ID:1964091

Oh, Mi-Jin; Choi, Hee-Don; Ha, Sang Keun; +2 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
arabinogalactan
frankincense
immunomodulation

Title: Isolation, structure elucidation, and immunostimulatory activity of polysaccharide fractions from Boswellia carterii frankincense resin

Citation Formats

References (36)

Similar Records

Related Subjects