Purification, Crystallization, and Preliminary Crystallographic Studies of Human As(III) S-Adenosylmethionine Methyltransferase (hAS3MT)

Radhakrishnan, M.; Packianathan, C.; Sankaran, B.; Kandavelu, P.; Rosen, B. P.

doi:10.1134/s1063774521070129

Title: Purification, Crystallization, and Preliminary Crystallographic Studies of Human As(III) S-Adenosylmethionine Methyltransferase (hAS3MT)

Journal Article · Tue Dec 14 00:00:00 EST 2021 · Crystallography Reports

DOI:https://doi.org/10.1134/s1063774521070129· OSTI ID:1924245

Radhakrishnan, M. ^[1]; Packianathan, C. ^[2]; Sankaran, B. ^[3]; Kandavelu, P. ^[4]; Rosen, B. P. ^[1]

Florida International Univ. (FIU), Miami, FL (United States)
Florida International Univ. (FIU), Miami, FL (United States); Texas A & M Univ., College Station, TX (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Univ. of Georgia, Athens, GA (United States)

Exposure to environmental arsenic is associated with serious of health issues such as cancer, diabetes and developmental delays in infants and children. In human liver, As(III) S-adenosylmethionine methyl transferase (hAS3MT) (EC 2.1.1.137) was proposed to be an detoxification process by methylation of inorganic arsenite into pentavalent methyl MAs(V) and dimethyl arsenite DMAs(V). More recently the first product was shown to be highly toxic and potentially carcinogenic trivalent methylarsenite (MAs(III)). Our studies are designed to elucidate the mechanism of AS3MT and its contribution to arsenic-related diseases. Here, in this paper, we report the first crystallization and preliminary X-ray diffraction analysis of the human AS3MT enzyme. The crystals belong to the monoclinic P12₁1 space group with unit cell parameters of a = 135.03 Å, b = 260.44 Å, c = 279.03 Å, α = 90.00°, β = 93.36°, γ = 90.00°.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); National Institutes of Health (NIH)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1924245

Journal Information:: Crystallography Reports, Vol. 66, Issue 7; ISSN 1063-7745

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

References (14)

Structure Determination of the ϕX174 Closed Procapsid Dokland, T.; McKenna, R.; Sherman, D. M. Acta Crystallographica Section D Biological Crystallography, Vol. 54, Issue 5 https://doi.org/10.1107/S0907444998002467	journal	September 1998
Arsenic detoxification and evolution of trimethylarsine gas by a microbial arsenite S -adenosylmethionine methyltransferase Qin, Jie; Rosen, Barry P.; Zhang, Yang Proceedings of the National Academy of Sciences, Vol. 103, Issue 7 https://doi.org/10.1073/pnas.0506836103	journal	February 2006
Protein crystallography for non-crystallographers, or how to get the best (but not more) from published macromolecular structures: Protein crystallography for non-crystallographers Wlodawer, Alexander; Minor, Wladek; Dauter, Zbigniew FEBS Journal, Vol. 275, Issue 1 https://doi.org/10.1111/j.1742-4658.2007.06178.x	journal	November 2007
Pathway of Human AS3MT Arsenic Methylation Dheeman, Dharmendra S.; Packianathan, Charles; Pillai, Jitesh K. Chemical Research in Toxicology, Vol. 27, Issue 11 https://doi.org/10.1021/tx500313k	journal	October 2014
Arsenic (+ 3 Oxidation State) Methyltransferase and the Methylation of Arsenicals in the Invertebrate Chordate Ciona intestinalis Thomas, David J.; Nava, Gerardo M.; Cai, Shi-Ying Toxicological Sciences, Vol. 113, Issue 1 https://doi.org/10.1093/toxsci/kfp250	journal	October 2009
Molecular replacement with MOLREP Vagin, Alexei; Teplyakov, Alexei Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 1 https://doi.org/10.1107/S0907444909042589	journal	December 2009
Pathways of arsenic uptake and efflux Garbinski, Luis D.; Rosen, Barry P.; Chen, Jian Environment International, Vol. 126 https://doi.org/10.1016/j.envint.2019.02.058	journal	May 2019
Low-Level Exposure to Arsenic in Drinking Water and Risk of Lung and Bladder Cancer: A Systematic Review and Dose–Response Meta-Analysis Boffetta, Paolo; Borron, Claire Dose-Response, Vol. 17, Issue 3 https://doi.org/10.1177/1559325819863634	journal	July 2019
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 Laemmli, U. K. Nature, Vol. 227, Issue 5259, p. 680-685 https://doi.org/10.1038/227680a0	journal	August 1970
The CCP4 suite programs for protein crystallography Acta Crystallographica Section D Biological Crystallography, Vol. 50, Issue 5, p. 760-763 https://doi.org/10.1107/S0907444994003112	journal	September 1994
XDS Kabsch, Wolfgang Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2 https://doi.org/10.1107/S0907444909047337	journal	January 2010
Earth Abides Arsenic Biotransformations Zhu, Yong-Guan; Yoshinaga, Masafumi; Zhao, Fang-Jie Annual Review of Earth and Planetary Sciences, Vol. 42, Issue 1 https://doi.org/10.1146/annurev-earth-060313-054942	journal	May 2014
Nonsynonymous Polymorphisms in the Human AS3MT Arsenic Methylation Gene: Implications for Arsenic Toxicity Li, Jiaojiao; Packianathan, Charles; Rossman, Toby G. Chemical Research in Toxicology, Vol. 30, Issue 7 https://doi.org/10.1021/acs.chemrestox.7b00113	journal	May 2017
Solvent content of protein crystals Matthews, B. W. Journal of Molecular Biology, Vol. 33, Issue 2 https://doi.org/10.1016/0022-2836(68)90205-2	journal	April 1968

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