Isothermal titration calorimetry of membrane protein interactions: FNR and the cytochrome b6f complex

Zakharov, Stanislav D.; Savikhin, Sergei; Misumi, Yuko; Kurisu, Genji; Cramer, William A.

doi:10.1016/j.bpj.2021.12.014

Title: Isothermal titration calorimetry of membrane protein interactions: FNR and the cytochrome b6f complex

Journal Article · Sat Jan 01 00:00:00 EST 2022 · Biophysical Journal

DOI:https://doi.org/10.1016/j.bpj.2021.12.014· OSTI ID:1909723

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Ferredoxin-NADP⁺ reductase (FNR) was previously inferred to bind to the cytochrome b₆f complex in the electron transport chain of oxygenic photosynthesis. In the present study, this inference has been examined through analysis of the thermodynamics of the interaction between FNR and the b₆f complex. Isothermal titration calorimetry (ITC) was used to characterize the physical interaction of FNR with b₆f complex derived from two plant sources (Spinacia oleracea and Zea maize). ITC did not detect a significant interaction of FNR with the b₆f complex in detergent solution nor with the complex reconstituted in liposomes. A previous inference of a small amplitude but defined FNR-b₆f interaction is explained by FNR interaction with micelles of the undecyl β-D maltoside (UDM) detergent micelles used to purify b₆f. Circular dichroism, employed to analyze the effect of detergent on the FNR structure, did not reveal significant changes in secondary or tertiary structures of FNR domains in the presence of UDM detergent. However, thermodynamic analysis implied a significant decrease in an interaction between the N-terminal FAD-binding and C-terminal NADP⁺-binding domains of FNR caused by detergent. The enthalpy, ΔH^o, and the entropy, ΔS^o, associated with FNR unfolding decreased four-fold in the presence of 1 mM UDM at pH 6.5. In addition to the conclusion regarding the absence of a binding interaction of significant amplitude between FNR and the b₆f complex, these studies provide a precedent for consideration of significant background protein-detergent interactions in ITC analyses involving integral membrane proteins.

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Research Organization:: Purdue Univ., West Lafayette, IN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division (CSGB); Ministry of Education, Culture, Sports, Science and Technology

Grant/Contract Number:: SC0018238; SC0018239; JP 16H06560

OSTI ID:: 1909723

Alternate ID(s):: OSTI ID: 1868618; OSTI ID: 1976894

Journal Information:: Biophysical Journal, Journal Name: Biophysical Journal Vol. 121 Journal Issue: 2; ISSN 0006-3495

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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