Sandwich Hybridization Assay for In Situ Real-Time Cyanobacterial Detection and Monitoring: A Review

Gong, Ping; Antrim, Anna K.; Bickman, Sarah R.; Cooley, Emily G.; Chung, Seung Ho

doi:10.3390/bios12080640

Title: Sandwich Hybridization Assay for In Situ Real-Time Cyanobacterial Detection and Monitoring: A Review

Journal Article · Sun Aug 14 00:00:00 EDT 2022 · Biosensors

DOI:https://doi.org/10.3390/bios12080640· OSTI ID:1905057

^[1];

^[2]; Bickman, Sarah R. ^[3]; Cooley, Emily G. ^[4];

^[4]

US Army Engineer Research and Development Center, Vicksburg, MS (United States)
Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)
LightDeck Diagnostics, Inc., Boulder, CO (United States)
Bennett Aerospace, Inc., Raleigh, NC (United States)

As cyanobacterial harmful algal bloom (cHAB) events increase in scale, severity, frequency, and duration around the world, rapid and accurate monitoring and characterization tools have become critically essential for regulatory and management decision-making. The composition of cHAB-forming cyanobacteria community can change significantly over time and space and be altered by sample preservation and transportation, making in situ monitoring necessary to obtain real-time and localized information. Sandwich hybridization assay (SHA) utilizes capture oligonucleotide probes for sensitive detection of target-specific nucleic acid sequences. As an amplification-free molecular biology technology, SHA can be adapted for in-situ, real-time or near real-time detection and qualitatively or semi-quantitatively monitoring of cHAB-forming cyanobacteria, owing to its characteristics such as being rapid, portable, inexpensive, and amenable to automation, high sensitivity, specificity and robustness, and multiplexing (i.e., detecting multiple targets simultaneously). Despite its successful application in the monitoring of marine and freshwater phytoplankton, there is still room for improvement. The ability to identify a cHAB community rapidly would decrease delays in cyanotoxin analyses, reduce costs, and increase sample throughput, allowing for timely actions to improve environmental and human health and the understanding of short- and long-term bloom dynamics. Real-time detection and quantitation of HAB-forming cyanobacteria is essential for improving environmental and public health and reducing associated costs. We review and propose to apply SHA for in situ cHABs monitoring.

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Research Organization:: Oak Ridge Inst. for Science and Education (ORISE), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC); USDOD

Grant/Contract Number:: SC0014664

OSTI ID:: 1905057

Journal Information:: Biosensors, Vol. 12, Issue 8; ISSN 2079-6374

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
cyanobacteria
harmful algal bloom (HAB)
sandwich hybridization assay (SHA)
nucleic acids
amplification-free
real-time in-situ monitoring
water quality
public health

Title: Sandwich Hybridization Assay for In Situ Real-Time Cyanobacterial Detection and Monitoring: A Review

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