Strategies for sample delivery for femtosecond crystallography

Martiel, Isabelle; Müller-Werkmeister, Henrike M.; Cohen, Aina E.

doi:10.1107/s2059798318017953

Title: Strategies for sample delivery for femtosecond crystallography

Journal Article · Fri Feb 01 00:00:00 EST 2019 · Acta Crystallographica. Section D. Structural Biology

DOI:https://doi.org/10.1107/s2059798318017953· OSTI ID:1903991

; Müller-Werkmeister, Henrike M.;

Highly efficient data-collection methods are required for successful macromolecular crystallography (MX) experiments at X-ray free-electron lasers (XFELs). XFEL beamtime is scarce, and the high peak brightness of each XFEL pulse destroys the exposed crystal volume. It is therefore necessary to combine diffraction images from a large number of crystals (hundreds to hundreds of thousands) to obtain a final data set, bringing about sample-refreshment challenges that have previously been unknown to the MX synchrotron community. In view of this experimental complexity, a number of sample delivery methods have emerged, each with specific requirements, drawbacks and advantages. To provide useful selection criteria for future experiments, this review summarizes the currently available sample delivery methods, emphasising the basic principles and the specific sample requirements. Two main approaches to sample delivery are first covered: (i) injector methods with liquid or viscous media and (ii) fixed-target methods using large crystals or using microcrystals inside multi-crystal holders or chips. Additionally, hybrid methods such as acoustic droplet ejection and crystal extraction are covered, which combine the advantages of both fixed-target and injector approaches.

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Research Organization:: SLAC National Accelerator Lab., Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource (SSRL)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC02-76SF00515; AC03-76SF00515

OSTI ID:: 1903991

Journal Information:: Acta Crystallographica. Section D. Structural Biology, Vol. 75, Issue 2; ISSN 2059-7983

Publisher:: International Union of Crystallography

Country of Publication:: United States

Language:: English

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Hydroxyethyl cellulose matrix applied to serial crystallography Sugahara, Michihiro; Nakane, Takanori; Masuda, Tetsuya Scientific Reports, Vol. 7, Article No. 703 https://doi.org/10.1038/s41598-017-00761-0	journal	April 2017
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