Functional and structural characterization of AntR, an Sb(III) responsive transcriptional repressor

Viswanathan, Thiruselvam; Chen, Jian; Wu, Minghan; An, Lijin; Kandavelu, Palani; Sankaran, Banumathi; Radhakrishnan, Manohar; Li, Mingshun; Rosen, Barry P.

doi:10.1111/mmi.14721

Title: Functional and structural characterization of AntR, an Sb(III) responsive transcriptional repressor

Journal Article · Wed Mar 31 00:00:00 EDT 2021 · Molecular Microbiology

DOI:https://doi.org/10.1111/mmi.14721· OSTI ID:1842462

Viswanathan, Thiruselvam ^[1]; Chen, Jian ^[1]; Wu, Minghan ^[2]; An, Lijin ^[2]; Kandavelu, Palani ^[3]; Sankaran, Banumathi ^[4]; Radhakrishnan, Manohar ^[1]; Li, Mingshun ^[2];

^[1]

Florida International Univ. (FIU), Miami, FL (United States)
Huazhong Agricultural Univ., Wuhan (China)
Univ. of Georgia, Athens, GA (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

The ant operon of the antimony-mining bacterium Comamonas testosterone JL40 confers resistance to Sb(III). The operon is transcriptionally regulated by the product of the first gene in the operon, antR. AntR is a member of ArsR/SmtB family of metal/metalloid-responsive repressors resistance. We purified and characterized C. testosterone AntR and demonstrated that it responds to metalloids in the order Sb(III) = methylarsenite (MAs(III) >> As(III)). The protein was crystallized, and the structure was solved at 2.1 Å resolution. The homodimeric structure of AntR adopts a classical ArsR/SmtB topology architecture. The protein has five cysteine residues, of which Cys103_a from one monomer and Cys113_b from the other monomer, are proposed to form one Sb(III) binding site, and Cys113_a and Cys103_b forming a second binding site. This is the first report of the structure and binding properties of a transcriptional repressor with high selectivity for environmental antimony.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: National Natural Science Foundation of China (NSFC); National Institutes of Health (NIH); USDOE Office of Science (SC), Basic Energy Sciences (BES); National Institute of General Medical Sciences (NIGMS); Howard Hughes Medical Institute

Grant/Contract Number:: AC02-05CH11231; 2016YFD0800702; 31970095; GM055425: GM136211: ES023779; W-31-109-Eng-38

OSTI ID:: 1842462

Journal Information:: Molecular Microbiology, Vol. 116, Issue 2; ISSN 0950-382X

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
antimony
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ArsR family
Comamonas testosterone
gene regulation
transcriptional repressor

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