Two Sides of a Coin: a Zika Virus Mutation Selected in Pregnant Rhesus Macaques Promotes Fetal Infection in Mice but at a Cost of Reduced Fitness in Nonpregnant Macaques and Diminished Transmissibility by Vectors

Lemos, Danilo; Stuart, Jackson B.; Louie, William; Singapuri, Anil; Ramírez, Ana L.; Watanabe, Jennifer; Usachenko, Jodie; Keesler, Rebekah I.; Sanchez-San Martin, Claudia; Li, Tony; Martyn, Calla; Oliveira, Glenn; Saraf, Sharada; Grubaugh, Nathan D.; Andersen, Kristian G.; Thissen, James; Allen, Jonathan; Borucki, Monica; Tsetsarkin, Konstantin A.; Pletnev, Alexander G.; Chiu, Charles Y.; Van Rompay, Koen K.  A.; Coffey, Lark L.

doi:10.1128/JVI.01605-20

Title: Two Sides of a Coin: a Zika Virus Mutation Selected in Pregnant Rhesus Macaques Promotes Fetal Infection in Mice but at a Cost of Reduced Fitness in Nonpregnant Macaques and Diminished Transmissibility by Vectors

Journal Article · Mon Nov 23 00:00:00 EST 2020 · Journal of Virology

DOI:https://doi.org/10.1128/JVI.01605-20· OSTI ID:1817081

Lemos, Danilo ^[1]; Stuart, Jackson B. ^[1]; Louie, William ^[1]; Singapuri, Anil ^[1]; Ramírez, Ana L. ^[1]; Watanabe, Jennifer ^[2]; Usachenko, Jodie ^[3]; Keesler, Rebekah I. ^[3]; Sanchez-San Martin, Claudia ^[4]; Li, Tony ^[4]; Martyn, Calla ^[4]; Oliveira, Glenn ^[5]; Saraf, Sharada ^[5]; Grubaugh, Nathan D. ^[6]; Andersen, Kristian G. ^[5]; Thissen, James ^[7]; Allen, Jonathan ^[7]; Borucki, Monica ^[7]; Tsetsarkin, Konstantin A. ^[8]; Pletnev, Alexander G. ^[8] more »

Univ. of California, Davis, CA (United States). School of Veterinary Medicine. Dept. of Pathology, Microbiology and Immunology
Univ. of California, Davis, CA (United States). California National Primate Research Center
Univ. of California, Davis, CA (United States). California National Primate Research Center
Univ. of California, San Francisco, CA (United States). Dept. of Lab. Medicine
Scripps Research Institute, San Diego, CA (United States)
Scripps Research Institute, San Diego, CA (United States); Yale Univ., New Haven, CT (United States). School of Public Health. Dept. of Epidemiology of Microbial Diseases
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
National Institutes of Health (NIH), Bethesda, MD (United States). National Institute of Allergy and Infectious Diseases. Lab. of Infectious Diseases

Although Zika virus infection of pregnant women can result in congenital Zika syndrome, the factors that cause the syndrome in some but not all infected mothers are still unclear. We identified a mutation that was present in some ZIKV genomes in experimentally inoculated pregnant rhesus macaques and their fetuses. Although we did not find an association between the presence of the mutation and fetal death, we performed additional studies with ZIKV with the mutation in nonpregnant macaques, pregnant mice, and mosquitoes. We observed that the mutation increased the ability of the virus to infect mouse fetuses but decreased its capacity to produce high levels of virus in the blood of nonpregnant macaques and to be transmitted by mosquitoes. This study shows that mutations in mosquito-borne viruses like ZIKV that increase fitness in pregnant vertebrates may not spread in outbreaks when they compromise transmission via mosquitoes and fitness in nonpregnant hosts.

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Research Organization:: Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC); National Institutes of Health (NIH); US Centers for Disease Control and Prevention (CDC)

Grant/Contract Number:: AC52-07NA27344; P51OD011107; 1R21AI129479-S; 1R01HL105704; 1R33AI129077; 1U01CK000516

OSTI ID:: 1817081

Journal Information:: Journal of Virology, Vol. 94, Issue 24; ISSN 0022-538X

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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Title: Two Sides of a Coin: a Zika Virus Mutation Selected in Pregnant Rhesus Macaques Promotes Fetal Infection in Mice but at a Cost of Reduced Fitness in Nonpregnant Macaques and Diminished Transmissibility by Vectors

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