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Title: Bioinformatics-aided identification, characterization and applications of mushroom linalool synthases

Journal Article · · Communications Biology
ORCiD logo [1];  [1];  [2];  [1]; ORCiD logo [3];  [4]
  1. Agency for Science, Technology and Research (A*STAR), Singapore (Singapore). Singapore Inst. of Food and Biotechnology Innovation (SIFBI)
  2. Agency for Science, Technology and Research (A*STAR), Singapore (Singapore). Bioinformatics Institute (BII)
  3. Agency for Science, Technology and Research (A*STAR), Singapore (Singapore). Bioinformatics Institute (BII); National Univ. of Singapore (Singapore). Dept. of Biological Sciences (DBS)
  4. Justus-Liebig Univ., Giessen (Germany). Inst. of Food Chemistry and Food Biotechnology
+ Show Author Affiliations

Enzymes empower chemical industries and are the keystone for metabolic engineering. For example, linalool synthases are indispensable for the biosynthesis of linalool, an important fragrance used in 60–80% cosmetic and personal care products. However, plant linalool synthases have low activities while expressed in microbes. Aided by bioinformatics analysis, four linalool/nerolidol synthases (LNSs) from various Agaricomycetes were accurately predicted and validated experimentally. Furthermore, we discovered a linalool synthase (Ap.LS) with exceptionally high levels of selectivity and activity from Agrocybe pediades, ideal for linalool bioproduction. It effectively converted glucose into enantiopure (R)-linalool in Escherichia coli, 44-fold and 287-fold more efficient than its bacterial and plant counterparts, respectively. Phylogenetic analysis indicated the divergent evolution paths for plant, bacterial and fungal linalool synthases. More critically, structural comparison provided catalytic insights into Ap.LS superior specificity and activity, and mutational experiments validated the key residues responsible for the specificity.

Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
1816330
Journal Information:
Communications Biology, Vol. 4, Issue 1; ISSN 2399-3642
Publisher:
Springer NatureCopyright Statement
Country of Publication:
United States
Language:
English

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59 BASIC BIOLOGICAL SCIENCES