Alternating magnetic fields and antibiotics eradicate biofilm on metal in a synergistic fashion

Wang, Qi; Vachon, Jonathan; Prasad, Bibin; Pybus, Christine A.; Lapin, Norman; Chopra, Rajiv; Greenberg, David E.

doi:10.1038/s41522-021-00239-y

Title: Alternating magnetic fields and antibiotics eradicate biofilm on metal in a synergistic fashion

Journal Article · Thu Aug 12 00:00:00 EDT 2021 · npj Biofilms and Microbiomes

DOI:https://doi.org/10.1038/s41522-021-00239-y· OSTI ID:1812961

;

; Prasad, Bibin; Pybus, Christine A.; Lapin, Norman;

;

Abstract Hundreds of thousands of human implant procedures require surgical revision each year due to infection. Infections are difficult to treat with conventional antibiotics due to the formation of biofilm on the implant surface. We have developed a noninvasive method to eliminate biofilm on metal implants using heat generated by intermittent alternating magnetic fields (iAMF). Here, we demonstrate that heat and antibiotics are synergistic in biofilm elimination. For Pseudomonas aeruginosa biofilm, bacterial burden was reduced >3 log with iAMF and ciprofloxacin after 24 h compared with either treatment alone ( p < 0.0001). This effect was not limited by pathogen or antibiotic as similar biofilm reductions were seen with iAMF and either linezolid or ceftriaxone in Staphylococcus aureus . iAMF and antibiotic efficacy was seen across various iAMF settings, including different iAMF target temperatures, dose durations, and dosing intervals. Initial mechanistic studies revealed membrane disruption as one factor important for AMF enhanced antibacterial activity in the biofilm setting. This study demonstrates the potential of utilizing a noninvasive approach to reduce biofilm off of metallic implants.

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Research Organization:: Univ. of Texas Southwestern Medical Center, Dallas, TX (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC), Office of SBIR/STTR Programs (SBIR/STTR)

Grant/Contract Number:: 1R44AI155291

OSTI ID:: 1812961

Alternate ID(s):: OSTI ID: 1904160

Journal Information:: npj Biofilms and Microbiomes, Journal Name: npj Biofilms and Microbiomes Vol. 7 Journal Issue: 1; ISSN 2055-5008

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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