Paraburkholderia solitsugae sp. nov. and Paraburkholderia elongata sp. nov., phenolic acid-degrading bacteria isolated from forest soil and emended description of Paraburkholderia madseniana

Journal Article · Tue Sep 01 00:00:00 EDT 2020 · International Journal of Systematic and Evolutionary Microbiology

DOI:https://doi.org/10.1099/ijsem.0.004387· OSTI ID:1802954

Wilhelm, Roland C. ^[1]; Cyle, K. Taylor  ^[1]; Martinez, Carmen Enid  ^[1]; Karasz, David C.  ^[1]; Newman, Jeffrey D.  ^[2]; Buckley, Daniel H.  ^[1]

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1. School of Integrative Plant Sciences, Bradfield Hall, Cornell University, Ithaca, NY, 14853, USA
2. Lycoming College, Williamsport, PA, 17701, USA

Two bacterial strains, 1N^Tand 5N^T, were isolated from hemlock forest soil using a soluble organic matter enrichment. Cells of 1N^T(0.65×1.85 µm) and 5N^T(0.6×1.85 µm) are Gram-stain-negative, aerobic, motile, non-sporulating and exist as single rods, diplobacilli or in chains of varying length. During growth in dilute media (≤0.1× tryptic soy broth; TSB), cells are primarily motile with flagella. At higher concentrations (≥0.3× TSB), cells of both strains increasingly form non-motile chains, and cells of 5N^Telongate (0.57×~7 µm) and form especially long filaments. Optimum growth of 1N^Tand 5N^Toccurred at 25–30 °C, pH 6.5–7.0 and <0.5% salinity. Results of comparative chemotaxonomic, genomic and phylogenetic analyses revealed that 1N^Tand 5N^Twere distinct from one another and their closest related type strains:Paraburkholderia madsenianaRP11^T,Paraburkholderia aspalathiLMG 27731^TandParaburkholderia caffeinilyticaCF1^T. The genomes of 1N^Tand 5N^Thad an average nucleotide identity (91.6 and 91.3%) andin silicoDNA–DNA hybridization values (45.8%±2.6 and 45.5%±2.5) and differed in functional gene content from their closest related type strains. The composition of fatty acids and patterns of substrate use, including the catabolism of phenolic acids, also differentiated strains 1N^Tand 5N^Tfrom each other and their closest relatives. The only ubiquinone present in strains 1N^Tand 5N^Twas Q-8. The major cellular fatty acids were C_{16 : 0}, 3OH-C_{16 : 0}, C_{17 : 0}cyclo, C_{19 : 0}cyclo ω8cand summed features 2 (3OH-C_{14 : 0}/ C_{16 : 1}iso I), 3 (C_{16 : 1}ω6c/ω7c) and 8 (C_{18 : 1}ω7c/ω6c). A third bacterium, strain RL16-012-BIC-B, was isolated from soil associated with shallow roots and was determined to be a strain ofP. madseniana(ANI, 98.8%; 16S rRNA gene similarity, 100%). Characterizations of strain RL16-012-BIC-B (DSM 110723=LMG 31706) led to proposed emendments to the species description ofP. madseniana. Our polyphasic approach demonstrated that strains 1N^Tand 5N^Trepresent novel species from the genusParaburkholderiafor which the namesParaburkholderia solitsugaesp. nov. (type strain 1N^T=DSM 110721^T=LMG 31704^T) andParaburkholderia elongatasp. nov. (type strain 5N^T=DSM 110722^T=LMG 31705^T) are proposed.

• Research Organization: Cornell Univ., Ithaca, NY (United States)
• Sponsoring Organization: USDOE Office of Science (SC)
• DOE Contract Number: SC0016364
• OSTI ID: 1802954
• Journal Information: International Journal of Systematic and Evolutionary Microbiology, Vol. 70, Issue 9; ISSN 1466-5026
• Publisher: International Union of Microbiological Societies
• Country of Publication: United States
• Language: English

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Microbiology