The Role of Titania Surface Coating by Atomic Layer Deposition in Improving Osteogenic Differentiation and Hard Tissue Formation of Dental Pulp Stem Cells

Chuang, Ya-Chen; Wang, Likun; Feng, Kuan-Che; Subramanian, Ashwanth; Chang, Chung-Chueh; Simon, Marcia; Nam, Chang-Yong; Rafailovich, Miriam

doi:10.1002/adem.202100097

Title: The Role of Titania Surface Coating by Atomic Layer Deposition in Improving Osteogenic Differentiation and Hard Tissue Formation of Dental Pulp Stem Cells

Journal Article · Sun May 16 00:00:00 EDT 2021 · Advanced Engineering Materials

DOI:https://doi.org/10.1002/adem.202100097· OSTI ID:1798491

^[1]; Wang, Likun ^[1]; Feng, Kuan-Che ^[1]; Subramanian, Ashwanth ^[1]; Chang, Chung-Chueh ^[1]; Simon, Marcia ^[1];

^[2]; Rafailovich, Miriam ^[1]

Stony Brook Univ., NY (United States)
Stony Brook Univ., NY (United States); Brookhaven National Lab. (BNL), Upton, NY (United States)

Recent studies have focused on modifying implant surface properties to improve bone regeneration and osseointegration. Herein, the role of titania surface coating in enabling dental pulp stem cells (DPSCs) differentiation and hard tissue formation is studied. Atomic layer deposition is applied at lower temperature to coat a nanolayer of titania onto hard and soft polybutadiene substrates without altering their moduli, to probe the effects of surface chemistry and substrate modulus. In the absence of titania, DPSCs prefer to attach and proliferate on the hard substrate, where particle-like biomineralization is present after 28 days in culture, whereas no biomineralization is observed on the soft substrate. Applying titania surface coating results in the improvement of adhesion and proliferation, regardless of substrate modulus, whereas cell and substrate moduli remain the same as control without titania coating. Furthermore, templated biomineralization is observed given the bone-like tissue composition by Raman analysis, in consistent with real time-polymerase chain reaction (RT-PCR) results showing upregulation of osteopontin (OPN) and bone sialoprotein (BSP), representing osteogenic differentiation. Furthermore, the results indicate that titania surface coating improved the ability to nucleate banded collagen fibers which in turn templated mineral deposits, and further induced the osteogenesis of DPSCs, regardless of its substrate modulus.

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Research Organization:: Brookhaven National Lab. (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); National Science Foundation (NSF)

Grant/Contract Number:: SC0012704; 1344267

OSTI ID:: 1798491

Report Number(s):: BNL-221704-2021-JAAM

Journal Information:: Advanced Engineering Materials, Vol. 23, Issue 9; ISSN 1438-1656

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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