Structural and genetic convergence of HIV-1 neutralizing antibodies in vaccinated non-human primates

Cai, Fangping; Chen, Wei-Hung; Wu, Weimin; Jones, Julia A.; Choe, Misook; Gohain, Neelakshi; Shen, Xiaoying; LaBranche, Celia; Eaton, Amanda; Sutherland, Laura; Lee, Esther M.; Hernandez, Giovanna E.; Wu, Nelson R.; Scearce, Richard; Seaman, Michael S.; Moody, M. Anthony; Santra, Sampa; Wiehe, Kevin; Tomaras, Georgia D.; Wagh, Kshitij; Korber, Bette; Bonsignori, Mattia; Montefiori, David C.; Haynes, Barton F.; de Val, Natalia; Joyce, M. Gordon; Saunders, Kevin O.; Burton, ed., Dennis R.

doi:10.1371/journal.ppat.1009624

Title: Structural and genetic convergence of HIV-1 neutralizing antibodies in vaccinated non-human primates

Journal Article · Fri Jun 04 00:00:00 EDT 2021 · PLoS Pathogens

DOI:https://doi.org/10.1371/journal.ppat.1009624· OSTI ID:1797877

Cai, Fangping;

; Wu, Weimin;

;

; Sutherland, Laura; Lee, Esther M.;

;

; Scearce, Richard;

;

; Wiehe, Kevin; Tomaras, Georgia D.; Wagh, Kshitij more »

A primary goal of HIV-1 vaccine development is the consistent elicitation of protective, neutralizing antibodies. While highly similar neutralizing antibodies (nAbs) have been isolated from multiple HIV-infected individuals, it is unclear whether vaccination can consistently elicit highly similar nAbs in genetically diverse primates. Here, we show in three outbred rhesus macaques that immunization with Env elicits a genotypically and phenotypically conserved nAb response. From these vaccinated macaques, we isolated four antibody lineages that had commonalities in immunoglobulin variable, diversity, and joining gene segment usage. Atomic-level structures of the antigen binding fragments of the two most similar antibodies showed nearly identical paratopes. The Env binding modes of each of the four vaccine-induced nAbs were distinct from previously known monoclonal HIV-1 neutralizing antibodies, but were nearly identical to each other. The similarities of these antibodies show that the immune system in outbred primates can respond to HIV-1 Env vaccination with a similar structural and genotypic solution for recognizing a particular neutralizing epitope. These results support rational vaccine design for HIV-1 that aims to reproducibly elicit, in genetically diverse primates, nAbs with specific paratope structures capable of binding conserved epitopes.

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Research Organization:: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOD; National Institutes of Health (NIH)

Grant/Contract Number:: W-31-109-Eng-38; AC02-06CH11357; 89233218CNA000001; W81XWH-07-2-0067; R01- AI120801; UM1-AI100645; 5P30 AI064518; HHSN261200800001E

OSTI ID:: 1797877

Alternate ID(s):: OSTI ID: 1786266; OSTI ID: 1827597

Report Number(s):: LA-UR-21-29267; 10.1371/journal.ppat.1009624

Journal Information:: PLoS Pathogens, Journal Name: PLoS Pathogens Vol. 17 Journal Issue: 6; ISSN 1553-7374

Publisher:: Public Library of Science (PLoS)Copyright Statement

Country of Publication:: United States

Language:: English

References (88)

Vaccine-Induced Antibodies that Neutralize Group 1 and Group 2 Influenza A Viruses Joyce, M. Gordon; Wheatley, Adam K.; Thomas, Paul V. Cell, Vol. 166, Issue 3 https://doi.org/10.1016/j.cell.2016.06.043	journal	July 2016
Limited Neutralizing Antibody Specificities Drive Neutralization Escape in Early HIV-1 Subtype C Infection Moore, Penny L.; Ranchobe, Nthabeleng; Lambson, Bronwen E. PLoS Pathogens, Vol. 5, Issue 9 https://doi.org/10.1371/journal.ppat.1000598	journal	September 2009
Multi-Donor Longitudinal Antibody Repertoire Sequencing Reveals the Existence of Public Antibody Clonotypes in HIV-1 Infection Setliff, Ian; McDonnell, Wyatt J.; Raju, Nagarajan Cell Host & Microbe, Vol. 23, Issue 6 https://doi.org/10.1016/j.chom.2018.05.001	journal	June 2018
Germline VRC01 antibody recognition of a modified clade C HIV-1 envelope trimer and a glycosylated HIV-1 gp120 core Borst, Andrew J.; Weidle, Connor E.; Gray, Matthew D. eLife, Vol. 7 https://doi.org/10.7554/eLife.37688	journal	November 2018
Commonality despite exceptional diversity in the baseline human antibody repertoire Briney, Bryan; Inderbitzin, Anne; Joyce, Collin Nature, Vol. 566, Issue 7744 https://doi.org/10.1038/s41586-019-0879-y	journal	January 2019
Broadly Neutralizing Antibody Responses in a Large Longitudinal Sub-Saharan HIV Primary Infection Cohort Landais, Elise; Huang, Xiayu; Havenar-Daughton, Colin PLOS Pathogens, Vol. 12, Issue 1 https://doi.org/10.1371/journal.ppat.1005369	journal	January 2016
Cooperation between somatic mutation and germline-encoded residues enables antibody recognition of HIV-1 envelope glycans Wu, Nelson R.; Nicely, Nathan I.; Lee, Esther M. PLOS Pathogens, Vol. 15, Issue 12 https://doi.org/10.1371/journal.ppat.1008165	journal	December 2019
N332-Directed Broadly Neutralizing Antibodies Use Diverse Modes of HIV-1 Recognition: Inferences from Heavy-Light Chain Complementation of Function Pancera, Marie; Yang, Yongping; Louder, Mark K. PLoS ONE, Vol. 8, Issue 2 https://doi.org/10.1371/journal.pone.0055701	journal	February 2013
The C3/465 glycan hole cluster in BG505 HIV-1 envelope is the major neutralizing target involved in preventing mucosal SHIV infection Charles, Tysheena P.; Burton, Samantha L.; Arunachalam, Prabhu S. PLOS Pathogens, Vol. 17, Issue 2 https://doi.org/10.1371/journal.ppat.1009257	journal	February 2021
Differential processing of HIV envelope glycans on the virus and soluble recombinant trimer Cao, Liwei; Pauthner, Matthias; Andrabi, Raiees Nature Communications, Vol. 9, Issue 1 https://doi.org/10.1038/s41467-018-06121-4	journal	September 2018
Protection of macaques against vaginal transmission of a pathogenic HIV-1/SIV chimeric virus by passive infusion of neutralizing antibodies Mascola, John R.; Stiegler, Gabriela; VanCott, Thomas C. Nature Medicine, Vol. 6, Issue 2 https://doi.org/10.1038/72318	journal	February 2000
Neutralization tiers of HIV-1 Montefiori, David C.; Roederer, Mario; Morris, Lynn Current Opinion in HIV and AIDS, Vol. 13, Issue 2 https://doi.org/10.1097/COH.0000000000000442	journal	January 2018
Structural Delineation of a Quaternary, Cleavage-Dependent Epitope at the gp41-gp120 Interface on Intact HIV-1 Env Trimers Blattner, Claudia; Lee, Jeong Hyun; Sliepen, Kwinten Immunity, Vol. 40, Issue 5 https://doi.org/10.1016/j.immuni.2014.04.008	journal	May 2014
Vaccine-Induced Protection from Homologous Tier 2 SHIV Challenge in Nonhuman Primates Depends on Serum-Neutralizing Antibody Titers Pauthner, Matthias G.; Nkolola, Joseph P.; Havenar-Daughton, Colin Immunity, Vol. 50, Issue 1 https://doi.org/10.1016/j.immuni.2018.11.011	journal	January 2019
Multiple roles for HIV broadly neutralizing antibodies Haynes, Barton F.; Burton, Dennis R.; Mascola, John R. Science Translational Medicine, Vol. 11, Issue 516 https://doi.org/10.1126/scitranslmed.aaz2686	journal	October 2019
Sequence and Structural Convergence of Broad and Potent HIV Antibodies That Mimic CD4 Binding Scheid, J. F.; Mouquet, H.; Ueberheide, B. Science, Vol. 333, Issue 6049 https://doi.org/10.1126/science.1207227	journal	July 2011
Isolation of a Monoclonal Antibody That Targets the Alpha-2 Helix of gp120 and Represents the Initial Autologous Neutralizing-Antibody Response in an HIV-1 Subtype C-Infected Individual Gray, E. S.; Moody, M. A.; Wibmer, C. K. Journal of Virology, Vol. 85, Issue 15 https://doi.org/10.1128/JVI.00563-11	journal	May 2011
Production of individualized V gene databases reveals high levels of immunoglobulin genetic diversity Corcoran, Martin M.; Phad, Ganesh E.; Bernat, Néstor Vázquez Nature Communications, Vol. 7, Issue 1 https://doi.org/10.1038/ncomms13642	journal	December 2016
High frequency of shared clonotypes in human B cell receptor repertoires Soto, Cinque; Bombardi, Robin G.; Branchizio, Andre Nature https://doi.org/10.1038/s41586-019-0934-8	journal	February 2019
The HIV-1 Envelope Glycoprotein C3/V4 Region Defines a Prevalent Neutralization Epitope following Immunization Lei, Lin; Yang, Yuhe R.; Tran, Karen Cell Reports, Vol. 27, Issue 2 https://doi.org/10.1016/j.celrep.2019.03.039	journal	April 2019
Epitope-based vaccine design yields fusion peptide-directed antibodies that neutralize diverse strains of HIV-1 Xu, Kai; Acharya, Priyamvada; Kong, Rui Nature Medicine, Vol. 24, Issue 6 https://doi.org/10.1038/s41591-018-0042-6	journal	June 2018
Envelope glycans of immunodeficiency virions are almost entirely oligomannose antigens Doores, K. J.; Bonomelli, C.; Harvey, D. J. Proceedings of the National Academy of Sciences, Vol. 107, Issue 31 https://doi.org/10.1073/pnas.1006498107	journal	July 2010
Antibody Light-Chain-Restricted Recognition of the Site of Immune Pressure in the RV144 HIV-1 Vaccine Trial Is Phylogenetically Conserved Wiehe, Kevin; Easterhoff, David; Luo, Kan Immunity, Vol. 41, Issue 6 https://doi.org/10.1016/j.immuni.2014.11.014	journal	December 2014
PHENIX : building new software for automated crystallographic structure determination Adams, Paul D.; Grosse-Kunstleve, Ralf W.; Hung, Li-Wei Acta Crystallographica Section D Biological Crystallography, Vol. 58, Issue 11 https://doi.org/10.1107/S0907444902016657	journal	October 2002
Immunogenicity of NYVAC Prime-Protein Boost Human Immunodeficiency Virus Type 1 Envelope Vaccination and Simian-Human Immunodeficiency Virus Challenge of Nonhuman Primates Saunders, Kevin O.; Santra, Sampa; Parks, Robert Journal of Virology, Vol. 92, Issue 8 https://doi.org/10.1128/JVI.02035-17	journal	February 2018
Antibody Lineages with Vaccine-Induced Antigen-Binding Hotspots Develop Broad HIV Neutralization Kong, Rui; Duan, Hongying; Sheng, Zizhang Cell, Vol. 178, Issue 3 https://doi.org/10.1016/j.cell.2019.06.030	journal	July 2019
What Are the Most Powerful Immunogen Design Vaccine Strategies?: Reverse Vaccinology 2.0 Shows Great Promise Burton, Dennis R. Cold Spring Harbor Perspectives in Biology, Vol. 9, Issue 11 https://doi.org/10.1101/cshperspect.a030262	journal	February 2017
Rational Design of Envelope Identifies Broadly Neutralizing Human Monoclonal Antibodies to HIV-1 Wu, X.; Yang, Z. -Y.; Li, Y. Science, Vol. 329, Issue 5993 https://doi.org/10.1126/science.1187659	journal	July 2010
Tiered Categorization of a Diverse Panel of HIV-1 Env Pseudoviruses for Assessment of Neutralizing Antibodies Seaman, M. S.; Janes, H.; Hawkins, N. Journal of Virology, Vol. 84, Issue 3 https://doi.org/10.1128/JVI.02108-09	journal	November 2009
Vaccine Elicitation of High Mannose-Dependent Neutralizing Antibodies against the V3-Glycan Broadly Neutralizing Epitope in Nonhuman Primates Saunders, Kevin O.; Nicely, Nathan I.; Wiehe, Kevin Cell Reports, Vol. 18, Issue 9 https://doi.org/10.1016/j.celrep.2017.02.003	journal	February 2017
Neutralizing antibodies to HIV-1 induced by immunization McCoy, Laura E.; Weiss, Robin A. The Journal of Experimental Medicine, Vol. 210, Issue 2 https://doi.org/10.1084/jem.20121827	journal	February 2013
Co-evolution of a broadly neutralizing HIV-1 antibody and founder virus Liao, Hua-Xin; Lynch, Rebecca; Zhou, Tongqing Nature, Vol. 496, Issue 7446 https://doi.org/10.1038/nature12053	journal	April 2013
Convergent Antibody Signatures in Human Dengue Parameswaran, Poornima; Liu, Yi; Roskin, Krishna M. Cell Host & Microbe, Vol. 13, Issue 6 https://doi.org/10.1016/j.chom.2013.05.008	journal	June 2013
Inhibition of Mammalian Glycan Biosynthesis Produces Non-self Antigens for a Broadly Neutralising, HIV-1 Specific Antibody Scanlan, Christopher N.; Ritchie, Gayle E.; Baruah, Kavitha Journal of Molecular Biology, Vol. 372, Issue 1 https://doi.org/10.1016/j.jmb.2007.06.027	journal	September 2007
Vaccine Induction of Heterologous Tier 2 HIV-1 Neutralizing Antibodies in Animal Models Saunders, Kevin O.; Verkoczy, Laurent K.; Jiang, Chuancang Cell Reports, Vol. 21, Issue 13 https://doi.org/10.1016/j.celrep.2017.12.028	journal	December 2017
Maturation Pathway from Germline to Broad HIV-1 Neutralizer of a CD4-Mimic Antibody Bonsignori, Mattia; Zhou, Tongqing; Sheng, Zizhang Cell, Vol. 165, Issue 2 https://doi.org/10.1016/j.cell.2016.02.022	journal	April 2016
IDLV-HIV-1 Env vaccination in non-human primates induces affinity maturation of antigen-specific memory B cells Blasi, Maria; Negri, Donatella; LaBranche, Celia Communications Biology, Vol. 1, Issue 1 https://doi.org/10.1038/s42003-018-0131-6	journal	September 2018
HIV-1 neutralizing antibodies induced by native-like envelope trimers Sanders, R. W.; van Gils, M. J.; Derking, R. Science, Vol. 349, Issue 6244 https://doi.org/10.1126/science.aac4223	journal	June 2015
Immunization expands B cells specific to HIV-1 V3 glycan in mice and macaques Escolano, Amelia; Gristick, Harry B.; Abernathy, Morgan E. Nature, Vol. 570, Issue 7762 https://doi.org/10.1038/s41586-019-1250-z	journal	May 2019
Isolation of a Human Anti-HIV gp41 Membrane Proximal Region Neutralizing Antibody by Antigen-Specific Single B Cell Sorting Morris, Lynn; Chen, Xi; Alam, Munir PLoS ONE, Vol. 6, Issue 9 https://doi.org/10.1371/journal.pone.0023532	journal	September 2011
Completeness of HIV-1 Envelope Glycan Shield at Transmission Determines Neutralization Breadth Wagh, Kshitij; Kreider, Edward F.; Li, Yingying Cell Reports, Vol. 25, Issue 4 https://doi.org/10.1016/j.celrep.2018.09.087	journal	October 2018
Public antibodies to malaria antigens generated by two LAIR1 insertion modalities Pieper, Kathrin; Tan, Joshua; Piccoli, Luca Nature, Vol. 548, Issue 7669 https://doi.org/10.1038/nature23670	journal	August 2017
Structure and immunogenicity of a stabilized HIV-1 envelope trimer based on a group-M consensus sequence Sliepen, Kwinten; Han, Byung Woo; Bontjer, Ilja Nature Communications, Vol. 10, Issue 1 https://doi.org/10.1038/s41467-019-10262-5	journal	May 2019
A Limited Number of Antibody Specificities Mediate Broad and Potent Serum Neutralization in Selected HIV-1 Infected Individuals Walker, Laura M.; Simek, Melissa D.; Priddy, Frances PLoS Pathogens, Vol. 6, Issue 8 https://doi.org/10.1371/journal.ppat.1001028	journal	August 2010
Structure and Diversity of the Rhesus Macaque Immunoglobulin Loci through Multiple De Novo Genome Assemblies Ramesh, Akshaya; Darko, Sam; Hua, Axin Frontiers in Immunology, Vol. 8 https://doi.org/10.3389/fimmu.2017.01407	journal	October 2017
Holes in the Glycan Shield of the Native HIV Envelope Are a Target of Trimer-Elicited Neutralizing Antibodies McCoy, Laura E.; van Gils, Marit J.; Ozorowski, Gabriel Cell Reports, Vol. 16, Issue 9 https://doi.org/10.1016/j.celrep.2016.07.074	journal	August 2016
Vaccine-Elicited Tier 2 HIV-1 Neutralizing Antibodies Bind to Quaternary Epitopes Involving Glycan-Deficient Patches Proximal to the CD4 Binding Site Crooks, Ema T.; Tong, Tommy; Chakrabarti, Bimal PLOS Pathogens, Vol. 11, Issue 5 https://doi.org/10.1371/journal.ppat.1004932	journal	May 2015
Glycan clustering stabilizes the mannose patch of HIV-1 and preserves vulnerability to broadly neutralizing antibodies Pritchard, Laura K.; Spencer, Daniel I. R.; Royle, Louise Nature Communications, Vol. 6, Issue 1 https://doi.org/10.1038/ncomms8479	journal	June 2015
Epitopes for neutralizing antibodies induced by HIV-1 envelope glycoprotein BG505 SOSIP trimers in rabbits and macaques Klasse, P. J.; Ketas, Thomas J.; Cottrell, Christopher A. PLOS Pathogens, Vol. 14, Issue 2 https://doi.org/10.1371/journal.ppat.1006913	journal	February 2018
Variable Loop Glycan Dependency of the Broad and Potent HIV-1-Neutralizing Antibodies PG9 and PG16 Doores, Katie J.; Burton, Dennis R. Journal of Virology, Vol. 84, Issue 20 https://doi.org/10.1128/JVI.00552-10	journal	August 2010
Magnitude and Breadth of the Neutralizing Antibody Response in the RV144 and Vax003 HIV-1 Vaccine Efficacy Trials Montefiori, D. C.; Karnasuta, C.; Huang, Y. Journal of Infectious Diseases, Vol. 206, Issue 3 https://doi.org/10.1093/infdis/jis367	journal	May 2012
Stoichiometry of Antibody Neutralization of Human Immunodeficiency Virus Type 1 Yang, Xinzhen; Kurteva, Svetla; Lee, Sandra Journal of Virology, Vol. 79, Issue 6 https://doi.org/10.1128/JVI.79.6.3500-3508.2005	journal	March 2005
Cooperation of B Cell Lineages in Induction of HIV-1-Broadly Neutralizing Antibodies Gao, Feng; Bonsignori, Mattia; Liao, Hua-Xin Cell, Vol. 158, Issue 3 https://doi.org/10.1016/j.cell.2014.06.022	journal	July 2014
MolProbity : all-atom structure validation for macromolecular crystallography Chen, Vincent B.; Arendall, W. Bryan; Headd, Jeffrey J. Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 1 https://doi.org/10.1107/S0907444909042073	journal	December 2009
Difficult-to-neutralize global HIV-1 isolates are neutralized by antibodies targeting open envelope conformations Han, Qifeng; Jones, Julia A.; Nicely, Nathan I. Nature Communications, Vol. 10, Issue 1 https://doi.org/10.1038/s41467-019-10899-2	journal	July 2019
Coot model-building tools for molecular graphics Emsley, Paul; Cowtan, Kevin Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12, p. 2126-2132 https://doi.org/10.1107/S0907444904019158	journal	November 2004
Broad and Potent Neutralizing Antibodies from an African Donor Reveal a New HIV-1 Vaccine Target Walker, L. M.; Phogat, S. K.; Chan-Hui, P. -Y. Science, Vol. 326, Issue 5950 https://doi.org/10.1126/science.1178746	journal	September 2009
Structural Constraints of Vaccine-Induced Tier-2 Autologous HIV Neutralizing Antibodies Targeting the Receptor-Binding Site Bradley, Todd; Fera, Daniela; Bhiman, Jinal Cell Reports, Vol. 14, Issue 1 https://doi.org/10.1016/j.celrep.2015.12.017	journal	January 2016
Development of the primary antibody repertoire Alt, F.; Blackwell, T.; Yancopoulos, G. Science, Vol. 238, Issue 4830 https://doi.org/10.1126/science.3317825	journal	November 1987
HIV-1 Vaccines Based on Antibody Identification, B Cell Ontogeny, and Epitope Structure Kwong, Peter D.; Mascola, John R. Immunity, Vol. 48, Issue 5 https://doi.org/10.1016/j.immuni.2018.04.029	journal	May 2018
A group M consensus envelope glycoprotein induces antibodies that neutralize subsets of subtype B and C HIV-1 primary viruses Liao, Hua-Xin; Sutherland, Laura L.; Xia, Shi-Mao Virology, Vol. 353, Issue 2, p. 268-282 https://doi.org/10.1016/j.virol.2006.04.043	journal	September 2006
The Glycan Shield of HIV Is Predominantly Oligomannose Independently of Production System or Viral Clade Bonomelli, Camille; Doores, Katie J.; Dunlop, D. Cameron PLoS ONE, Vol. 6, Issue 8 https://doi.org/10.1371/journal.pone.0023521	journal	August 2011
Glycosylation Site-Specific Analysis of HIV Envelope Proteins (JR-FL and CON-S) Reveals Major Differences in Glycosylation Site Occupancy, Glycoform Profiles, and Antigenic Epitopesʼ Accessibility Go, Eden P.; Irungu, Janet; Zhang, Ying Journal of Proteome Research, Vol. 7, Issue 4 https://doi.org/10.1021/pr7006957	journal	April 2008
The C3-V4 Region Is a Major Target of Autologous Neutralizing Antibodies in Human Immunodeficiency Virus Type 1 Subtype C Infection Moore, Penny L.; Gray, Elin S.; Choge, Isaac A. Journal of Virology, Vol. 82, Issue 4 https://doi.org/10.1128/JVI.02187-07	journal	December 2007
IMGT®, the international ImMunoGeneTics information system® 25 years on Lefranc, Marie-Paule; Giudicelli, Véronique; Duroux, Patrice Nucleic Acids Research, Vol. 43, Issue D1 https://doi.org/10.1093/nar/gku1056	journal	November 2014
Magnitude and Breadth of a Nonprotective Neutralizing Antibody Response in an Efficacy Trial of a Candidate HIV‐1 gp120 Vaccine Gilbert, Peter; Wang, Maggie; Wrin, Terri The Journal of Infectious Diseases, Vol. 202, Issue 4 https://doi.org/10.1086/654816	journal	August 2010
Elicitation of Robust Tier 2 Neutralizing Antibody Responses in Nonhuman Primates by HIV Envelope Trimer Immunization Using Optimized Approaches Pauthner, Matthias; Havenar-Daughton, Colin; Sok, Devin Immunity, Vol. 46, Issue 6 https://doi.org/10.1016/j.immuni.2017.05.007	journal	June 2017
Unliganded HIV-1 gp120 core structures assume the CD4-bound conformation with regulation by quaternary interactions and variable loops Kwon, Y. D.; Finzi, A.; Wu, X. Proceedings of the National Academy of Sciences, Vol. 109, Issue 15 https://doi.org/10.1073/pnas.1112391109	journal	March 2012
Recurrent Variable Region Gene Usage and Somatic Mutation in the Human Antibody Response to the Capsular Polysaccharide of Streptococcus pneumoniae Type 23F Zhou, J.; Lottenbach, K. R.; Barenkamp, S. J. Infection and Immunity, Vol. 70, Issue 8 https://doi.org/10.1128/IAI.70.8.4083-4091.2002	journal	August 2002
Somatic generation of antibody diversity Tonegawa, Susumu Nature, Vol. 302, Issue 5909 https://doi.org/10.1038/302575a0	journal	April 1983
Quantification of the Impact of the HIV-1-Glycan Shield on Antibody Elicitation Zhou, Tongqing; Doria-Rose, Nicole A.; Cheng, Cheng Cell Reports, Vol. 19, Issue 4 https://doi.org/10.1016/j.celrep.2017.04.013	journal	April 2017
Structural Repertoire of HIV-1-Neutralizing Antibodies Targeting the CD4 Supersite in 14 Donors Zhou, Tongqing; Lynch, Rebecca M.; Chen, Lei Cell, Vol. 161, Issue 6 https://doi.org/10.1016/j.cell.2015.05.007	journal	June 2015
B-cell–lineage immunogen design in vaccine development with HIV-1 as a case study Haynes, Barton F.; Kelsoe, Garnett; Harrison, Stephen C. Nature Biotechnology, Vol. 30, Issue 5 https://doi.org/10.1038/nbt.2197	journal	May 2012
Reconstructing a B-cell clonal lineage. I. Statistical inference of unobserved ancestors Kepler, Thomas B. F1000Research, Vol. 2 https://doi.org/10.12688/f1000research.2-103.v1	journal	January 2013
Cryo-EM structure of a native, fully glycosylated, cleaved HIV-1 envelope trimer Lee, J. H.; Ozorowski, G.; Ward, A. B. Science, Vol. 351, Issue 6277 https://doi.org/10.1126/science.aad2450	journal	March 2016
Developmental pathway for potent V1V2-directed HIV-neutralizing antibodies Doria-Rose, Nicole A.; Schramm, Chaim A.; Gorman, Jason Nature, Vol. 509, Issue 7498 https://doi.org/10.1038/nature13036	journal	March 2014
Prevalence of broadly neutralizing antibody responses during chronic HIV-1 infection Hraber, Peter; Seaman, Michael S.; Bailer, Robert T. AIDS, Vol. 28, Issue 2 https://doi.org/10.1097/QAD.0000000000000106	journal	January 2014
Centralized immunogens as a vaccine strategy to overcome HIV-1 diversity Gao, F.; Korber, B. T.; Weaver, Eric Expert Review of Vaccines, Vol. 3, Issue sup1 https://doi.org/10.1586/14760584.3.4.S161	journal	August 2004
Focused Evolution of HIV-1 Neutralizing Antibodies Revealed by Structures and Deep Sequencing Wu, X.; Zhou, T.; Zhu, J. Science, Vol. 333, Issue 6049 https://doi.org/10.1126/science.1207532	journal	August 2011
Well-Ordered Trimeric HIV-1 Subtype B and C Soluble Spike Mimetics Generated by Negative Selection Display Native-like Properties Guenaga, Javier; de Val, Natalia; Tran, Karen PLoS Pathogens, Vol. 11, Issue 1 https://doi.org/10.1371/journal.ppat.1004570	journal	January 2015
IMGT, the international ImMunoGeneTics database Lefranc, M. -P.; Giudicelli, V.; Ginestoux, C. Nucleic Acids Research, Vol. 27, Issue 1 https://doi.org/10.1093/nar/27.1.209	journal	January 1999
Automated electron microscope tomography using robust prediction of specimen movements Mastronarde, David N. Journal of Structural Biology, Vol. 152, Issue 1 https://doi.org/10.1016/j.jsb.2005.07.007	journal	October 2005
Multidonor Analysis Reveals Structural Elements, Genetic Determinants, and Maturation Pathway for HIV-1 Neutralization by VRC01-Class Antibodies Zhou, Tongqing; Zhu, Jiang; Wu, Xueling Immunity, Vol. 39, Issue 2 https://doi.org/10.1016/j.immuni.2013.04.012	journal	August 2013
[20] Processing of X-ray diffraction data collected in oscillation mode Otwinowski, Zbyszek; Minor, Wladek Macromolecular Crystallography Part A, 307-326 https://doi.org/10.1016/S0076-6879(97)76066-X	book	January 1997
Comprehensive Cross-Clade Neutralization Analysis of a Panel of Anti-Human Immunodeficiency Virus Type 1 Monoclonal Antibodies Binley, James M.; Wrin, Terri; Korber, Bette Journal of Virology, Vol. 78, Issue 23 https://doi.org/10.1128/JVI.78.23.13232-13252.2004	journal	December 2004
Diversity Considerations in HIV-1 Vaccine Selection Gaschen, Brian; Taylor, Jesse; Yusim, Karina Science, Vol. 296, Issue 5577, p. 2354-2360 https://doi.org/10.1126/science.1070441	journal	June 2002
An MPER antibody neutralizes HIV-1 using germline features shared among donors Zhang, Lei; Irimia, Adriana; He, Lingling Nature Communications, Vol. 10, Issue 1 https://doi.org/10.1038/s41467-019-12973-1	journal	November 2019
Human neutralizing monoclonal antibodies of the IgG1 subtype protect against mucosal simian–human immunodeficiency virus infection Baba, Timothy W.; Liska, Vladimir; Hofmann-Lehmann, Regina Nature Medicine, Vol. 6, Issue 2 https://doi.org/10.1038/72309	journal	February 2000

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