Genome‐enabled exploration of microbial ecology and evolution in the sea: a rising tide lifts all boats

DeLong, Edward F.

doi:10.1111/1462-2920.15403

Title: Genome‐enabled exploration of microbial ecology and evolution in the sea: a rising tide lifts all boats

Journal Article · Tue Feb 02 00:00:00 EST 2021 · Environmental Microbiology

DOI:https://doi.org/10.1111/1462-2920.15403· OSTI ID:1786944

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Daniel K. Inouye Centre for Microbial Oceanography: Research and Education, University of Hawaii, Manoa Honolulu HI 96822 USA

Summary As a young bacteriologist just launching my career during the early days of the ‘microbial revolution’ in the 1980s, I was fortunate to participate in some early discoveries, and collaborate in the development of cross‐disciplinary methods now commonly referred to as "metagenomics". My early scientific career focused on applying phylogenetic and genomic approaches to characterize ‘wild’ bacteria, archaea and viruses in their natural habitats, with an emphasis on marine systems. These central interests have not changed very much for me over the past three decades, but knowledge, methodological advances and new theoretical perspectives about the microbial world certainly have. In this invited ‘How we did it’ perspective, I trace some of the trajectories of my lab's collective efforts over the years, including phylogenetic surveys of microbial assemblages in marine plankton and sediments, development of microbial community gene‐ and genome‐enabled surveys, and application of genome‐guided, cultivation‐independent functional characterization of novel enzymes, pathways and their relationships to in situ biogeochemistry. Throughout this short review, I attempt to acknowledge, all the mentors, students, postdocs and collaborators who enabled this research. Inevitably, a brief autobiographical review like this cannot be fully comprehensive, so sincere apologies to any of my great colleagues who are not explicitly mentioned herein. I salute you all as well!

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Research Organization:: Univ. of Hawaii at Manoa, Honolulu, HI (United States)

Sponsoring Organization:: USDOE; Simons Foundation; Gordon and Betty Moore Foundation (GBMF); Agouron Institute; David and Lucille Packard Foundation; Monterey Bay Aquarium Research Institute; National Science Foundation (NSF); USEPA; National Oceanic and Atmospheric Administration (NOAA); US Department of the Navy, Office of Naval Research (ONR)

Grant/Contract Number:: 721223; 6000

OSTI ID:: 1786944

Alternate ID(s):: OSTI ID: 1786945; OSTI ID: 1816763

Journal Information:: Environmental Microbiology, Journal Name: Environmental Microbiology Vol. 23 Journal Issue: 3; ISSN 1462-2912

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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