Reduced type-A carbohydrate-binding module interactions to cellulose I leads to improved endocellulase activity

Nemmaru, Bhargava; Ramirez, Nicholas; Farino, Cindy J.; Yarbrough, John M.; Kravchenko, Nicholas; Chundawat, Shishir S.

doi:10.1002/bit.27637

Title: Reduced type-A carbohydrate-binding module interactions to cellulose I leads to improved endocellulase activity

Journal Article · Wed Dec 16 00:00:00 EST 2020 · Biotechnology and Bioengineering

DOI:https://doi.org/10.1002/bit.27637· OSTI ID:1762453

^[1]; Ramirez, Nicholas ^[1]; Farino, Cindy J. ^[1]; Yarbrough, John M. ^[2]; Kravchenko, Nicholas ^[1];

^[1]

Rutgers Univ., Piscataway, NJ (United States)
National Renewable Energy Lab. (NREL), Golden, CO (United States)

Dissociation of nonproductively bound cellulolytic enzymes from cellulose is hypothesized to be a key rate-limiting factor impeding cost-effective biomass conversion to fermentable sugars. However, the role of carbohydrate-binding modules (CBMs) in enabling nonproductive enzyme binding is not well understood. Here, we examine the subtle interplay of CBM binding and cellulose hydrolysis activity for three models type-A CBMs (Families 1, 3a, and 64) tethered to multifunctional endoglucanase (CelE) on two distinct cellulose allomorphs (i.e., cellulose I and III). We generated a small library of mutant CBMs with varying cellulose affinity, as determined by equilibrium binding assays, followed by monitoring cellulose hydrolysis activity of CelE–CBM fusion constructs. Finally, kinetic binding assays using quartz crystal microbalance with dissipation were employed to measure CBM adsorption and desorption rate constants kon and koff, respectively, towards nanocrystalline cellulose derived from both allomorphs. Overall, our results indicate that reduced CBM equilibrium binding affinity towards cellulose I alone, resulting from increased desorption rates (koff) and reduced effective adsorption rates (nkon), is correlated to overall improved endocellulase activity. Future studies could employ similar approaches to unravel the role of CBMs in nonproductive enzyme binding and develop improved cellulolytic enzymes for industrial applications.

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Research Organization:: National Renewable Energy Lab. (NREL), Golden, CO (United States); USDOE Bioenergy Research Centers (BRC) (United States). Great Lakes Bioenergy Research Center (GLBRC)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office; National Science Foundation (NSF); USDOE Office of Science (SC)

Grant/Contract Number:: AC36-08GO28308; 1604421; 1846797; FC02-07ER64494

OSTI ID:: 1762453

Report Number(s):: NREL/JA-2700-78358; MainId:32275; UUID:1784f455-c1a4-478e-aa3c-93f9b6be829f; MainAdminID:19163

Journal Information:: Biotechnology and Bioengineering, Vol. 118, Issue 3; ISSN 0006-3592

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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nonproductive binding
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quartz crystal microbalance with dissipation

Title: Reduced type-A carbohydrate-binding module interactions to cellulose I leads to improved endocellulase activity

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