Rapid and Efficient Genetic Transformation of Sorghum via Agrobacterium-Mediated Method

Journal Article · Fri Oct 12 00:00:00 EDT 2018 · Current Protocols in Plant Biology

DOI:https://doi.org/10.1002/cppb.20077· OSTI ID:1633419

Do, Phat Tien ^[1]; Lee, Hyeyoung ^[2]; Nelson-Vasilchik, Kimberly ^[3]; Kausch, Albert ^[3]; Zhang, Zhanyuan J. ^[2]

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1. Univ. of Missouri, Columbia, MO (United States); Vietnam Academy of Science and Technology, Hanoi (Vietnam)
2. Univ. of Missouri, Columbia, MO (United States)
3. Univ. of Rhode Island, Kingston, RI (United States)

Genetic transformation via Agrobacterium-mediated methodology has been used in many sorghum studies. However, the transformation efficiency still varies significantly due to high dependence on sorghum genotypes and technical expertise. In this article, we describe a sorghum transformation procedure in sufficient detail using a public genotype, P898012. This system utilizes a standard binary transgenic vector carrying the bar gene as a selectable marker and immature embryos as starting explants. Glufosinate is employed as the selective agent during callus and shoot induction. Further, this procedure is relatively rapid, efficient, highly reproducible, and should be applicable for many other sorghum genotypes

• Research Organization: Univ. of Rhode Island, Kingston, RI (United States)
• Sponsoring Organization: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF)
• DOE Contract Number: SC0018277
• OSTI ID: 1633419
• Journal Information: Current Protocols in Plant Biology, Vol. 3, Issue 4; ISSN 2379-8068
• Publisher: Wiley
• Country of Publication: United States
• Language: English

Related Subjects

Agrobacterium-mediated
genetic transformation
P898012
sorghum
standard binary vector