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Title: Genome sequence of the Medicago-nodulating Ensifer meliloti commercial inoculant strain RRI128

Journal Article · · Standards in Genomic Sciences
DOI:https://doi.org/10.4056/sigs.4929626· OSTI ID:1628688
 [1];  [2];  [2];  [1];  [3];  [4];  [5];  [5];  [5];  [6];  [6];  [6];  [6];  [5];  [5];  [7];  [7]
  1. Murdoch Univ., WA (Australia). Centre for Rhizobium Studies
  2. South Australian Research and Development Inst., Urrbrae, SA (Australia)
  3. Deakin Univ., Victoria (Australia). School of Life and Environmental Sciences
  4. Los Alamos National Lab. (LANL), Los Alamos, NM (United States). Biosciences Division
  5. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
  6. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biological Data Management and Technology Center
  7. Los Alamos National Lab. (LANL), Los Alamos, NM (United States). Bioscience Division
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Ensifer meliloti strain RRI128 is an aerobic, motile, Gram-negative, non-spore-forming rod. RRI128 was isolated from a nodule recovered from the roots of barrel medic (Medicago truncatula) grown in the greenhouse and inoculated with soil collected from Victoria, Australia. The strain is used in commercial inoculants in Australia. RRI128 nodulates and forms an effective symbiosis with a diverse range of lucerne cultivars (Medicago sativa) and several species of annual medic (M. truncatula, Medicago littoralis and Medicago tornata), but forms an ineffective symbiosis with Medicago polymorpha. Here we describe the features of E. meliloti strain RRI128, together with genome sequence information and annotation. The 6,900,273 bp draft genome is arranged into 156 scaffolds of 157 contigs, contains 6,683 protein-coding genes and 87 RNA-only encoding genes, and is one of 100 rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project.

Research Organization:
Los Alamos National Laboratory (LANL), Los Alamos, NM (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
Grant/Contract Number:
AC52-06NA25396; AC02-05CH11231; AC52-07NA27344
OSTI ID:
1628688
Journal Information:
Standards in Genomic Sciences, Vol. 9, Issue 3; ISSN 1944-3277
Publisher:
BioMed CentralCopyright Statement
Country of Publication:
United States
Language:
English

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Measurements of carbon utilization by single bacterial species in sterile soil: insights into Rhizobium spp. journal June 2016

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Genetics & Heredity
Microbiology
root-nodule bacteria
nitrogen fixation
rhizobia
Alphaproteobacteria