Metatranscriptomics and Amplicon Sequencing Reveal Mutualisms in Seagrass Microbiomes

Crump, Byron C.; Wojahn, John M.; Tomas, Fiona; Mueller, Ryan S.

doi:10.3389/fmicb.2018.00388

Title: Metatranscriptomics and Amplicon Sequencing Reveal Mutualisms in Seagrass Microbiomes

Journal Article · Thu Mar 15 00:00:00 EDT 2018 · Frontiers in Microbiology

DOI:https://doi.org/10.3389/fmicb.2018.00388· OSTI ID:1628164

Crump, Byron C. ^[1]; Wojahn, John M. ^[1]; Tomas, Fiona ^[2]; Mueller, Ryan S. ^[3]

Oregon State Univ., Corvallis, OR (United States). College of Earth, Ocean and Atmospheric Sciences
Oregon State Univ., Corvallis, OR (United States). Dept. of Fisheries and Wildlife; Universitat de les Illes Balears (UIB), Esporles (Spain). Instituto Mediterraneo de Estudios Avanzados (IMEDEA). Consejo Superior de Investigaciones Cientificas (CSIC)
Oregon State Univ., Corvallis, OR (United States). Dept. of Mircobiology

Terrestrial plants benefit from many well-understood mutualistic relationships with root and leaf-associated microbiomes, but relatively little is known about these relationships for seagrass and other aquatic plants. We used 16S rRNA gene amplicon sequencing and metatranscriptomics to assess potential mutualisms between microorganisms and the seagrasses Zostera marina and Zostera japonica collected from mixed beds in Netarts Bay, OR, United States. The phylogenetic composition of leaf-, root-, and water column-associated bacterial communities were strikingly different, but these communities were not significantly different between plant species. Many taxa present on leaves were related to organisms capable of consuming the common plant metabolic waste product methanol, and of producing agarases, which can limit the growth of epiphytic algae. Taxa present on roots were related to organisms capable of oxidizing toxic sulfur compounds and of fixing nitrogen. Metatranscriptomic sequencing identified expression of genes involved in all of these microbial metabolic processes at levels greater than typical water column bacterioplankton, and also identified expression of genes involved in denitrification and in bacterial synthesis of the plant growth hormone indole-3-acetate. These results provide the first evidence using metatranscriptomics that seagrass microbiomes carry out a broad range of functions that may benefit their hosts, and imply that microbe–plant mutualisms support the health and growth of aquatic plants.

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Research Organization:: Oregon State Univ., Corvallis, OR (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1628164

Journal Information:: Frontiers in Microbiology, Vol. 9; ISSN 1664-302X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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Tomato RNA-seq Data Mining Reveals the Taxonomic and Functional Diversity of Root-Associated Microbiota Chialva, Matteo; Ghignone, Stefano; Novero, Mara Microorganisms, Vol. 8, Issue 1 https://doi.org/10.3390/microorganisms8010038	journal	December 2019
Regional and Microenvironmental Scale Characterization of the Zostera muelleri Seagrass Microbiome Hurtado-McCormick, Valentina; Kahlke, Tim; Petrou, Katherina Frontiers in Microbiology, Vol. 10 https://doi.org/10.3389/fmicb.2019.01011	journal	May 2019
Climate Change Impacts on Seagrass Meadows and Macroalgal Forests: An Integrative Perspective on Acclimation and Adaptation Potential Duarte, Bernardo; Martins, Irene; Rosa, Rui Frontiers in Marine Science, Vol. 5 https://doi.org/10.3389/fmars.2018.00190	journal	June 2018

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59 BASIC BIOLOGICAL SCIENCES
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