Proteomic Analysis of Cellular Response Induced by Multi-Walled Carbon Nanotubes Exposure in A549 Cells

Ju, Li; Zhang, Guanglin; Zhang, Xing; Jia, Zhenyu; Gao, Xiangjing; Jiang, Ying; Yan, Chunlan; Duerksen-Hughes, Penelope J.; Chen, Fanqing Frank; Li, Hongjuan; Zhu, Xinqiang; Yang, Jun

doi:10.1371/journal.pone.0084974

Title: Proteomic Analysis of Cellular Response Induced by Multi-Walled Carbon Nanotubes Exposure in A549 Cells

Journal Article · Tue Jan 14 00:00:00 EST 2014 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0084974· OSTI ID:1627671

Ju, Li ^[1]; Zhang, Guanglin ^[1]; Zhang, Xing ^[1]; Jia, Zhenyu ^[1]; Gao, Xiangjing ^[1]; Jiang, Ying ^[1]; Yan, Chunlan ^[1]; Duerksen-Hughes, Penelope J. ^[2]; Chen, Fanqing Frank ^[3]; Li, Hongjuan ^[4]; Zhu, Xinqiang ^[1]; Yang, Jun ^[1]

Zhejiang University, Hangzhou (China)
Loma Linda University School of Medicine, Loma Linda, CA (United States)
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Hangzhou Normal University, Hangzhou (China)

The wide application of multi-walled carbon nanotubes (MWCNT) has raised serious concerns about their safety on human health and the environment. However, the potential harmful effects of MWCNT remain unclear and contradictory. To clarify the potentially toxic effects of MWCNT and to elucidate the associated underlying mechanisms, the effects of MWCNT on human lung adenocarcinoma A549 cells were examined at both the cellular and the protein level. Cytotoxicity and genotoxicity were examined, followed by a proteomic analysis (2-DE coupled with LC-MS/MS) of the cellular response to MWCNT. Our results demonstrate that MWCNT induces cytotoxicity in A549 cells only at relatively high concentrations and longer exposure time. Within a relatively low dosage range (30 mg/ml) and short time period (24 h), MWCNT treatment does not induce significant cytotoxicity, cell cycle changes, apoptosis, or DNA damage. However, at these low doses and times, MWCNT treatment causes significant changes in protein expression. A total of 106 proteins show altered expression at various time points and dosages, and of these, 52 proteins were further identified by MS. Identified proteins are involved in several cellular processes including proliferation, stress, and cellular skeleton organization. In particular, MWCNT treatment causes increases in actin expression. This increase has the potential to contribute to increased migration capacity and may be mediated by reactive oxygen species (ROS).

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Natural Science Foundation of China (NSFC); Zhejiang Provincial Natural Science Foundation; Zhejiang Provincial Department of Science and Technology; Zhejiang Medical Support Plan in Discipline Construction

Grant/Contract Number:: AC02-05CH11231; 81172692; 81202241; 81373036; 81302398; LY12H26006; 2013C14016; 11-ZC02

OSTI ID:: 1627671

Journal Information:: PLoS ONE, Vol. 9, Issue 1; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
apoptosis
carbon nanotubes
actins
DNA-binding proteins
cytoskeletal proteins
cell cycle and cell division
cytotoxicity
DNA damage

Title: Proteomic Analysis of Cellular Response Induced by Multi-Walled Carbon Nanotubes Exposure in A549 Cells

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