Genome sequence of Ensifer medicae Di28; an effective N2-fixing microsymbiont of Medicago murex and M. polymorpha

Garau, Giovanni; Terpolilli, Jason; Hill, Yvette; Tian, Rui; Howieson, John; Bräu, Lambert; Goodwin, Lynne; Han, James; Reddy, TBK; Huntemann, Marcel; Pati, Amrita; Woyke, Tanja; Mavromatis, Konstantinos; Markowitz, Victor; Ivanova, Natalia; Kyrpides, Nikos; Reeve, Wayne

doi:10.1186/1944-3277-9-4

Title: Genome sequence of Ensifer medicae Di28; an effective N2-fixing microsymbiont of Medicago murex and M. polymorpha

Journal Article · Wed Jan 01 00:00:00 EST 2014 · Standards in Genomic Sciences

DOI:https://doi.org/10.1186/1944-3277-9-4· OSTI ID:1626670

Garau, Giovanni ^[1]; Terpolilli, Jason ^[2]; Hill, Yvette ^[2]; Tian, Rui ^[2]; Howieson, John ^[2]; Bräu, Lambert ^[3]; Goodwin, Lynne ^[4]; Han, James ^[5]; Reddy, TBK ^[5]; Huntemann, Marcel ^[5]; Pati, Amrita ^[6]; Woyke, Tanja ^[5]; Mavromatis, Konstantinos ^[6]; Markowitz, Victor ^[6]; Ivanova, Natalia ^[4]; Kyrpides, Nikos ^[7]; Reeve, Wayne ^[2]

Univ. of Sassari (Italy). Dipartimento di Agraria. S.T.A.A.
Murdoch Univ., WA (Australia). Centre for Rhizobium Studies
Deakin Univ., VIC (Australia). School of Life and Environmental Sciences
Los Alamos National Lab. (LANL), Los Alamos, NM (United States). Biosciences Division
USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biological Data Management and Technology Center
Los Alamos National Lab. (LANL), Los Alamos, NM (United States). Biosciences Division; King Abdulaziz Univ., Jeddah (Saudi Arabia). Dept. of Biological Sciences

Ensifer medicae Di28 is an aerobic, motile, Gram-negative, non-spore-forming rod that can exist as a soil saprophyte or as a legume microsymbiont of Medicago spp. Di28 was isolated in 1998 from a nodule recovered from the roots of M. polymorpha growing in the south east of Sardinia (Italy). Di28 is an effective microsymbiont of the annual forage legumes M. polymorpha and M. murex and is capable of establishing a partially effective symbiotic association with the perennial M. sativa. Here we describe the features of E. medicae Di28, together with genome sequence information and its annotation. The 6,553,624 bp standard draft genome is arranged into 104 scaffolds of 104 contigs containing 6,394 protein-coding genes and 75 RNA-only encoding genes. This rhizobial genome is one of 100 sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project.

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Research Organization:: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: AC52-06NA25396; AC02-05CH11231; AC52-07NA27344

OSTI ID:: 1626670

Journal Information:: Standards in Genomic Sciences, Vol. 9, Issue 1; ISSN 1944-3277

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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Title: Genome sequence of Ensifer medicae Di28; an effective N2-fixing microsymbiont of Medicago murex and M. polymorpha

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