The Nonmuscle Myosin Regulatory Light Chain Gene mlc-4 Is Required for Cytokinesis, Anterior-Posterior Polarity, and Body Morphology during Caenorhabditis elegans Embryogenesis

Shelton, Christopher A.; Carter, J. Clayton; Ellis, Gregory C.; Bowerman, Bruce

doi:10.1083/jcb.146.2.439

Title: The Nonmuscle Myosin Regulatory Light Chain Gene mlc-4 Is Required for Cytokinesis, Anterior-Posterior Polarity, and Body Morphology during Caenorhabditis elegans Embryogenesis

Journal Article · Mon Jul 26 00:00:00 EDT 1999 · Journal of Cell Biology

DOI:https://doi.org/10.1083/jcb.146.2.439· OSTI ID:1625113

Shelton, Christopher A. ^[1]; Carter, J. Clayton ^[1]; Ellis, Gregory C. ^[1]; Bowerman, Bruce ^[1]

Univ. of Oregon, Eugene, OR (United States). Inst. of Molecular Biology

Using RNA-mediated genetic interference in a phenotypic screen, we identified a conserved nonmuscle myosin II regulatory light chain gene in Caenorhabditis elegans, which we name mlc-4. Maternally supplied mlc-4 function is required for cytokinesis during both meiosis and mitosis and for establishment of anterior-posterior (a-p) asymmetries after fertilization. Reducing the function of mlc-4 or nmy-2, a nonmuscle myosin II gene, also leads to a loss of polarized cytoplasmic flow in the C. elegans zygote, supporting models in which cytoplasmic flow may be required to establish a-p differences. Germline P granule localization at the time of cytoplasmic flow is also lost in these embryos, although P granules do become localized to the posterior pole after the first mitosis. This result suggests that a mechanism other than cytoplasmic flow or mlc-4/nmy-2 activity can generate some a-p asymmetries in the C. elegans zygote. By isolating a deletion allele, we show that removing zygotic mlc-4 function results in an elongation phenotype during embryogenesis. An mlc-4/green fluorescent protein transgene is expressed in lateral rows of hypodermal cells and these cells fail to properly change shape in mlc-4 mutant animals during elongation.

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Research Organization:: Univ. of Oregon, Eugene, OR (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

OSTI ID:: 1625113

Journal Information:: Journal of Cell Biology, Vol. 146, Issue 2; ISSN 0021-9525

Publisher:: Rockefeller University PressCopyright Statement

Country of Publication:: United States

Language:: English

References (46)

Cortical and cytoplasmic flow polarity in early embryonic cells of Caenorhabditis elegans. Hird, S. N.; White, J. G. Journal of Cell Biology, Vol. 121, Issue 6 https://doi.org/10.1083/jcb.121.6.1343	journal	June 1993
Role of citron kinase as a target of the small GTPase Rho in cytokinesis Madaule, Pascal; Eda, Masatoshi; Watanabe, Naoki Nature, Vol. 394, Issue 6692 https://doi.org/10.1038/28873	journal	July 1998
Caenorhabditis elegans LET-502 is related to Rho-binding kinases and human myotonic dystrophy kinase and interacts genetically with a homolog of the regulatory subunit of smooth muscle myosin phosphatase to affect cell shape. Wissmann, A.; Ingles, J.; McGhee, J. D. Genes & Development, Vol. 11, Issue 4 https://doi.org/10.1101/gad.11.4.409	journal	February 1997
Asymmetrically distributed PAR-3 protein contributes to cell polarity and spindle alignment in early C. elegans embryos Etemad-Moghadam, Bijan; Guo, Su; Kemphues, Kenneth J. Cell, Vol. 83, Issue 5 https://doi.org/10.1016/0092-8674(95)90187-6	journal	December 1995
Chapter 13 Blastomere Culture and Analysis Edgar, Lois G. Cuenorhubditis elegans: Modern Biologcal Analysis of an Organism https://doi.org/10.1016/S0091-679X(08)61393-X	book	January 1995
THE GENETICS OF CAENORHABDITIS ELEGANS Brenner, S. Genetics, Vol. 77, Issue 1 https://doi.org/10.1093/genetics/77.1.71	journal	May 1974
Three-dimensional structure of myosin subfragment-1: a molecular motor Rayment, I.; Rypniewski, W.; Schmidt-Base, K. Science, Vol. 261, Issue 5117 https://doi.org/10.1126/science.8316857	journal	July 1993
pos-1 encodes a cytoplasmic zinc-finger protein essential for germline specification in C. elegans Tabara, H.; Hill, R. J.; Mello, C. C. Development, Vol. 126, Issue 1 https://doi.org/10.1242/dev.126.1.1	journal	January 1999
Formation of the first cleavage spindle in nematode embryos Albertson, Donna G. Developmental Biology, Vol. 101, Issue 1 https://doi.org/10.1016/0012-1606(84)90117-9	journal	January 1984
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans Fire, Andrew; Xu, SiQun; Montgomery, Mary K. Nature, Vol. 391, Issue 6669 https://doi.org/10.1038/35888	journal	February 1998
A Putative Catenin–Cadherin System Mediates Morphogenesis of the Caenorhabditis elegans Embryo Costa, Michael; Raich, William; Agbunag, Cristina The Journal of Cell Biology, Vol. 141, Issue 1 https://doi.org/10.1083/jcb.141.1.297	journal	April 1998
Segregation of germ granules in living Caenorhabditis elegans embryos: cell-type-specific mechanisms for cytoplasmic localisation Hird, S. N.; Paulsen, J. E.; Strome, S. Development, Vol. 122, Issue 4 https://doi.org/10.1242/dev.122.4.1303	journal	April 1996
Myosin Light Chain–activating Phosphorylation Sites Are Required for Oogenesis in Drosophila Jordan, Pascale; Karess, Roger Journal of Cell Biology, Vol. 139, Issue 7 https://doi.org/10.1083/jcb.139.7.1805	journal	December 1997
PAR-2 is asymmetrically distributed and promotes association of P granules and PAR-1 with the cortex in C. elegans embryos Boyd, L.; Guo, S.; Levitan, D. Development, Vol. 122, Issue 10 https://doi.org/10.1242/dev.122.10.3075	journal	October 1996
Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences. Mello, C. C.; Kramer, J. M.; Stinchcomb, D. The EMBO Journal, Vol. 10, Issue 12 https://doi.org/10.1002/j.1460-2075.1991.tb04966.x	journal	December 1991
Genes critical for muscle development and function in Caenorhabditis elegans identified through lethal mutations Williams, Bd; Waterston, Rh Journal of Cell Biology, Vol. 124, Issue 4 https://doi.org/10.1083/jcb.124.4.475	journal	February 1994
A highly conserved centrosomal kinase, AIR-1, is required for accurate cell cycle progression and segregation of developmental factors in Caenorhabditis elegans embryos Schumacher, J. M.; Ashcroft, N.; Donovan, P. J. Development, Vol. 125, Issue 22 https://doi.org/10.1242/dev.125.22.4391	journal	November 1998
Time-dependent responses to glp-1-mediated inductions in early C. elegans embryos Shelton, C. A.; Bowerman, B. Development, Vol. 122, Issue 7 https://doi.org/10.1242/dev.122.7.2043	journal	July 1996
Centrosome dynamics in early embryos of Caenorhabditis elegans Keating, H. H.; White, J. G. Journal of Cell Science, Vol. 111, Issue 20 https://doi.org/10.1242/jcs.111.20.3027	journal	October 1998
Cell Fusions in the Developing Epithelia of C. elegans Podbilewicz, Benjamin; White, John G. Developmental Biology, Vol. 161, Issue 2 https://doi.org/10.1006/dbio.1994.1041	journal	February 1994
An analysis of the role of microfilaments in the establishment and maintenance of asymmetry in Caenorhabditis elegans zygotes Hill, David P.; Strome, Susan Developmental Biology, Vol. 125, Issue 1 https://doi.org/10.1016/0012-1606(88)90060-7	journal	January 1988
A novel family of genes encoding putative pheromone receptors in mammals Dulac, Catherine; Axel, Richard Cell, Vol. 83, Issue 2 https://doi.org/10.1016/0092-8674(95)90161-2	journal	October 1995
The dynactin complex is required for cleavage plane specification in early Caenorhabditis elegans embryos Skop, Ahna R.; White, John G. Current Biology, Vol. 8, Issue 20 https://doi.org/10.1016/S0960-9822(98)70465-8	journal	October 1998
Generation of asymmetry and segregation of germ-line granules in early C. elegans embryos Strome, Susan; Wood, William B. Cell, Vol. 35, Issue 1 https://doi.org/10.1016/0092-8674(83)90203-9	journal	November 1983
Reverse genetics by chemical mutagenesis in Caenorhabditis elegans Jansen, Gert; Hazendonk, Esther; Thijssen, Karen L. Nature Genetics, Vol. 17, Issue 1 https://doi.org/10.1038/ng0997-119	journal	September 1997
Green fluorescent protein as a marker for gene expression Chalfie, M.; Tu, Y.; Euskirchen, G. Science, Vol. 263, Issue 5148 https://doi.org/10.1126/science.8303295	journal	February 1994
The regulatory light chain of nonmuscle myosin is encoded by spaghetti-squash, a gene required for cytokinesis in Drosophila Karess, Roger E.; Chang, Xiao-jia; Edwards, Kevin A. Cell, Vol. 65, Issue 7 https://doi.org/10.1016/0092-8674(91)90013-O	journal	June 1991
Mutations in the par genes of Caenorhabditis elegans affect cytoplasmic reorganization during the first cell cycle Kirby, Colleen; Kusch, Meredith; Kemphues, Kenneth Developmental Biology, Vol. 142, Issue 1 https://doi.org/10.1016/0012-1606(90)90164-E	journal	November 1990
AIR-2: An Aurora/Ipl1-related Protein Kinase Associated with Chromosomes and Midbody Microtubules Is Required for Polar Body Extrusion and Cytokinesis in Caenorhabditis elegans Embryos Schumacher, Jill M.; Golden, Andy; Donovan, Peter J. Journal of Cell Biology, Vol. 143, Issue 6 https://doi.org/10.1083/jcb.143.6.1635	journal	December 1998
Chromatin silencing and the maintenance of a functional germline in Caenorhabditis elegans Kelly, W. G.; Fire, A. Development, Vol. 125, Issue 13 https://doi.org/10.1242/dev.125.13.2451	journal	July 1998
Functions of the Caenorhabditis elegans Regulatory Myosin Light Chain Genes mlc-1 and mlc-2 Rushforth, Alice M.; White, Claudia Cummins; Anderson, Philip Genetics, Vol. 150, Issue 3 https://doi.org/10.1093/genetics/150.3.1067	journal	November 1998
The Role of Actin Filaments in Patterning theCaenorhabditis elegansCuticle Costa, Michael; Draper, Bruce W.; Priess, James R. Developmental Biology, Vol. 184, Issue 2 https://doi.org/10.1006/dbio.1997.8530	journal	April 1997
Identification of genes required for cytoplasmic localization in early C. elegans embryos Kemphues, Kenneth J.; Priess, James R.; Morton, Diane G. Cell, Vol. 52, Issue 3 https://doi.org/10.1016/S0092-8674(88)80024-2	journal	February 1988
Caenorhabditis elegans morphogenesis: The role of the cytoskeleton in elongation of the embryo Priess, James R.; Hirsh, David I. Developmental Biology, Vol. 117, Issue 1 https://doi.org/10.1016/0012-1606(86)90358-1	journal	September 1986
Rho-associated Kinase Directly Induces Smooth Muscle Contraction through Myosin Light Chain Phosphorylation Kureishi, Yasuko; Kobayashi, Sei; Amano, Mutsuki Journal of Biological Chemistry, Vol. 272, Issue 19 https://doi.org/10.1074/jbc.272.19.12257	journal	May 1997
Specification of the anteroposterior axis in Caenorhabditis elegans Goldstein, B.; Hird, S. N. Development, Vol. 122, Issue 5 https://doi.org/10.1242/dev.122.5.1467	journal	May 1996
A non-muscle myosin required for embryonic polarity in Caenorhabditis elegans Guo, Su; Kemphues, Kenneth J. Nature, Vol. 382, Issue 6590 https://doi.org/10.1038/382455a0	journal	August 1996
Wnt Signaling and an APC-Related Gene Specify Endoderm in Early C. elegans Embryos Rocheleau, Christian E.; Downs, William D.; Lin, Rueyling Cell, Vol. 90, Issue 4 https://doi.org/10.1016/S0092-8674(00)80531-0	journal	August 1997
The embryonic cell lineage of the nematode Caenorhabditis elegans Sulston, J. E.; Schierenberg, E.; White, J. G. Developmental Biology, Vol. 100, Issue 1 https://doi.org/10.1016/0012-1606(83)90201-4	journal	November 1983
Genome Sequence of the Nematode C. elegans: A Platform for Investigating Biology Science, Vol. 282, Issue 5396 https://doi.org/10.1126/science.282.5396.2012	journal	December 1998
The identification of a Caenorhabditis elegans homolog of p34cdc2 kinase Mori, Hiro; Palmer, Robert E.; Sternberg, Paul W. Molecular and General Genetics MGG, Vol. 245, Issue 6 https://doi.org/10.1007/bf00297285	journal	November 1994
A modular set of lacZ fusion vectors for studying gene expression in Caenorhabditis elegans Fire, Andrew; Harrison, Susan White; Dixon, Dennis Gene, Vol. 93, Issue 2 https://doi.org/10.1016/0378-1119(90)90224-f	journal	January 1990
Use of dsRNA-Mediated Genetic Interference to Demonstrate that frizzled and frizzled 2 Act in the Wingless Pathway Kennerdell, Jason R.; Carthew, Richard W. Cell, Vol. 95, Issue 7 https://doi.org/10.1016/s0092-8674(00)81725-0	journal	December 1998
Actin dynamics in vivo Welch, M. Current Opinion in Cell Biology, Vol. 9, Issue 1 https://doi.org/10.1016/s0955-0674(97)80152-4	journal	January 1997
Molecular mechanisms of nonmuscle myosin-II regulation Bresnick, Anne R. Current Opinion in Cell Biology, Vol. 11, Issue 1 https://doi.org/10.1016/s0955-0674(99)80004-0	journal	February 1999
A nematode kinesin required for cleavage furrow advancement Powers, James; Bossinger, Olaf; Rose, Debra Current Biology, Vol. 8, Issue 20 https://doi.org/10.1016/s0960-9822(98)70470-1	journal	October 1998

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Iwasa, Hiroaki; Maimaiti, Sainawaer; Kuroyanagi, Hidehito; +6 more

Nonmuscle myosin IIA and IIB differentially contribute to intrinsic and directed migration of human embryonic lung fibroblasts

Journal Article · Thu Mar 15 00:00:00 EDT 2018 · Biochemical and Biophysical Research Communications · OSTI ID:1625113

Kuragano, Masahiro; Murakami, Yota; Takahashi, Masayuki

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59 BASIC BIOLOGICAL SCIENCES
cell biology
microfilament proteins
RNA-mediated
interference
nonmuscle myosin II regulatory light chain
P granules
morphogenesis

Title: The Nonmuscle Myosin Regulatory Light Chain Gene mlc-4 Is Required for Cytokinesis, Anterior-Posterior Polarity, and Body Morphology during Caenorhabditis elegans Embryogenesis

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