The eukaryotic CMG helicase pumpjack and integration into the replisome

Sun, Jingchuan; Yuan, Zuanning; Georgescu, Roxanna; Li, Huilin; O'Donnell, Mike

doi:10.1080/19491034.2016.1174800

Title: The eukaryotic CMG helicase pumpjack and integration into the replisome

Journal Article · Tue Mar 01 00:00:00 EST 2016 · Nucleus

DOI:https://doi.org/10.1080/19491034.2016.1174800· OSTI ID:1625101

Sun, Jingchuan ^[1]; Yuan, Zuanning ^[2]; Georgescu, Roxanna ^[3]; Li, Huilin ^[4]; O'Donnell, Mike ^[3]

Brookhaven National Lab. (BNL), Upton, NY (United States). Biology Dept.
State Univ. of New York (SUNY), Stony Brook, NY (United States). Dept. of Biochemistry & Cell Biology
Rockefeller Univ., New York, NY (United States). Howard Hughes Medical Inst. DNA Replication Lab.
Brookhaven National Lab. (BNL), Upton, NY (United States). Biology Dept.; State Univ. of New York (SUNY), Stony Brook, NY (United States). Dept. of Biochemistry & Cell Biology

The eukaryotic replisome is a multiprotein machine that contains DNA polymerases, sliding clamps, helicase, and primase along with several factors that participate in cell cycle and checkpoint control. The detailed structure of the 11-subunit CMG helicase (Cdc45/Mcm2-7/GINS) has been solved recently by cryoEM single-particle 3D reconstruction and reveals pumpjack motions that imply an unexpected mechanism of DNA translocation. CMG is also the organizing center of the replisome. Recent in vitro reconstitution of leading and lagging strand DNA synthesis has enabled structural analysis of the replisome. By building the replisome in stages from pure proteins, single-particle EM studies have identified the overall architecture of the eukaryotic replisome. Suprisingly leading and lagging strand polymerases bind to opposite faces of the CMG helicase, unlike the long-held view that DNA polymerases are located in back of the helicase to act on the unwound strands.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-98CH10886; NIH [GM111472, OD12272, GM115809]

OSTI ID:: 1625101

Journal Information:: Nucleus, Vol. 7, Issue 2; ISSN 1949-1034

Publisher:: Taylor & FrancisCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 8 works

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