A Site of Vulnerability on the Influenza Virus Hemagglutinin Head Domain Trimer Interface

Bangaru, Sandhya; Lang, Shanshan; Schotsaert, Michael; Vanderven, Hillary A.; Zhu, Xueyong; Kose, Nurgun; Bombardi, Robin; Finn, Jessica A.; Kent, Stephen J.; Gilchuk, Pavlo; Gilchuk, Iuliia; Turner, Hannah L.; García-Sastre, Adolfo; Li, Sheng; Ward, Andrew B.; Wilson, Ian A.; Crowe, Jr., James E.

doi:10.1016/j.cell.2019.04.011

Title: A Site of Vulnerability on the Influenza Virus Hemagglutinin Head Domain Trimer Interface

Journal Article · Wed May 01 00:00:00 EDT 2019 · Cell

DOI:https://doi.org/10.1016/j.cell.2019.04.011· OSTI ID:1619329

Bangaru, Sandhya; Lang, Shanshan; Schotsaert, Michael; Vanderven, Hillary A.; Zhu, Xueyong; Kose, Nurgun; Bombardi, Robin; Finn, Jessica A.; Kent, Stephen J.; Gilchuk, Pavlo; Gilchuk, Iuliia; Turner, Hannah L.; García-Sastre, Adolfo; Li, Sheng; Ward, Andrew B.; Wilson, Ian A.; Crowe, Jr., James E.

Here, we describe the discovery of a naturally occurring human antibody (Ab), FluA-20, that recognizes a new site of vulnerability on the hemagglutinin (HA) head domain and reacts with most influenza A viruses. Structural characterization of FluA-20 with H1 and H3 head domains revealed a novel epitope in the HA trimer interface, suggesting previously unrecognized dynamic features of the trimeric HA protein. The critical HA residues recognized by FluA-20 remain conserved across most subtypes of influenza A viruses, which explains the Ab’s extraordinary breadth. The Ab rapidly disrupted the integrity of HA protein trimers, inhibited cell-to-cell spread of virus in culture, and protected mice against challenge with viruses of H1N1, H3N2, H5N1, or H7N9 subtypes when used as prophylaxis or therapy. The FluA-20 Ab has uncovered an exceedingly conserved protective determinant in the influenza HA head domain trimer interface that is an unexpected new target for anti-influenza therapeutics and vaccines.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS); SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource (SSRL)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-76SF00515; AC02-06CH11357

OSTI ID:: 1619329

Alternate ID(s):: OSTI ID: 1513049; OSTI ID: 1836722

Journal Information:: Cell, Journal Name: Cell Vol. 177 Journal Issue: 5; ISSN 0092-8674

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 115 works

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59 BASIC BIOLOGICAL SCIENCES
influenza A virus
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viral
B-lymphocytes
antigen-antibody reactions
monoclonal
antibody-dependent cell cytotoxicity
hemagglutinin glycoproteins
influenza virus

Title: A Site of Vulnerability on the Influenza Virus Hemagglutinin Head Domain Trimer Interface

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