Phenotypic characterization and comparative transcriptomics of evolved Saccharomyces cerevisiae strains with improved tolerance to lignocellulosic derived inhibitors

Thompson, Olivia A.; Hawkins, Gary M.; Gorsich, Steven W.; Doran-Peterson, Joy

doi:10.1186/s13068-016-0614-y

Title: Phenotypic characterization and comparative transcriptomics of evolved Saccharomyces cerevisiae strains with improved tolerance to lignocellulosic derived inhibitors

Journal Article · Tue Sep 20 00:00:00 EDT 2016 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-016-0614-y· OSTI ID:1618649

; Hawkins, Gary M.; Gorsich, Steven W.; Doran-Peterson, Joy

Background: Lignocellulosic biomass continues to be investigated as a viable source for bioethanol production. However, the pretreatment process generates inhibitory compounds that impair the growth and fermentation performance of microorganisms such as Saccharomyces cerevisiae. Pinewood specifically has been shown to be challenging in obtaining industrially relevant ethanol titers. An industrial S. cerevisiae strain was subjected to directed evolution and adaptation in pretreated pine biomass and resultant strains, GHP1 and GHP4, exhibited improved growth and fermentative ability on pretreated pine in the presence of related inhibitory compounds. A comparative transcriptomic approach was applied to identify and characterize differences in phenotypic stability of evolved strains. Results: Evolved strains displayed different fermentative capabilities with pretreated pine that appear to be influenced by the addition or absence of 13 inhibitory compounds during pre-culturing. GHP4 performance was consistent independent of culturing conditions, while GHP1 performance was dependent on culturing with inhibitors. Comparative transcriptomics revealed 52 genes potentially associated with stress responses to multiple inhibitors simultaneously. Fluorescence microscopy revealed improved cellular integrity of both strains with mitochondria exhibiting resistance to the damaging effects of inhibitors in contrast to the parent. Conclusions: Multiple potentially novel genetic targets have been discovered for understanding stress tolerance through the characterization of our evolved strains. This study specifically examines the synergistic effects of multiple inhibitors and identified targets will guide future studies in remediating effects of inhibitors and further development of robust yeast strains for multiple industrial applications.

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Research Organization:: Univ. of Georgia, Athens, GA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: DOE-EE000410; SC0010687

OSTI ID:: 1618649

Alternate ID(s):: OSTI ID: 1626976

Journal Information:: Biotechnology for Biofuels, Journal Name: Biotechnology for Biofuels Vol. 9 Journal Issue: 1; ISSN 1754-6834

Publisher:: Springer Science + Business MediaCopyright Statement

Country of Publication:: Netherlands

Language:: English

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Cited by: 30 works

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59 BASIC BIOLOGICAL SCIENCES
Biotechnology & Applied Microbiology
Energy & Fuels
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Phenotypic characterization and comparative transcriptomics of evolved Saccharomyces cerevisiae strains with improved tolerance to lignocellulosic derived inhibitors Thompson, Olivia; Hawkins, Gary; Gorsich, Steven https://doi.org/10.6084/m9.figshare.c.3629723 The current record is supplemented by this collection	collection	January 2016
Phenotypic characterization and comparative transcriptomics of evolved Saccharomyces cerevisiae strains with improved tolerance to lignocellulosic derived inhibitors Thompson, Olivia; Hawkins, Gary; Gorsich, Steven https://doi.org/10.6084/m9.figshare.c.3629723.v1 The current record is supplemented by this collection	collection	January 2016
MOESM1 of Phenotypic characterization and comparative transcriptomics of evolved Saccharomyces cerevisiae strains with improved tolerance to lignocellulosic derived inhibitors Thompson, Olivia; Hawkins, Gary; Gorsich, Steven https://doi.org/10.6084/M9.FIGSHARE.C.3629723_D1 The current record is supplemented by this dataset	dataset	January 2016
MOESM1 of Phenotypic characterization and comparative transcriptomics of evolved Saccharomyces cerevisiae strains with improved tolerance to lignocellulosic derived inhibitors Thompson, Olivia; Hawkins, Gary; Gorsich, Steven https://doi.org/10.6084/m9.figshare.c.3629723_d1.v1 The current record is supplemented by this dataset	dataset	January 2016
MOESM2 of Phenotypic characterization and comparative transcriptomics of evolved Saccharomyces cerevisiae strains with improved tolerance to lignocellulosic derived inhibitors Thompson, Olivia; Hawkins, Gary; Gorsich, Steven https://doi.org/10.6084/m9.figshare.c.3629723_d2.v1 The current record is supplemented by this text	text	January 2016