Effect of iron and silica nanoparticles’ size on in vitro human skin binding and penetration

Jung, Eui; Hui, Xiaoying; Zhu, Hanjiang; Zhang, Alissa; Wang, Wei; Buchholz, Bruce; Maibach, Howard

doi:10.1177/2397847319893054

Title: Effect of iron and silica nanoparticles’ size on in vitro human skin binding and penetration

Journal Article · Fri Dec 27 00:00:00 EST 2019 · Toxicology Research and Application

DOI:https://doi.org/10.1177/2397847319893054· OSTI ID:1603236

Jung, Eui ^[1];

^[1]; Zhu, Hanjiang ^[1]; Zhang, Alissa ^[1]; Wang, Wei ^[2]; Buchholz, Bruce ^[3]; Maibach, Howard ^[1]

Univ. of California San Francisco, CA (United States)
Aramco Services Company, Cambridge, MA (United States)
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

This in vitro skin study determined absorption, diffusion, and binding rates of four [14C]-labeled nanoparticles (NPs): 12 nm Fe₃O₄, 32 nm Fe₃O₄@SiO₂, 33 nm SiO₂, and 78 nm SiO₂in each layer of human cadaver skin. In vitro microdialysis device and flow-through skin diffusion system were used to measure the binding affinity to the stratum corneum (SC) and permeability into/through skin layer of the four NPs with different physical–chemical properties, respectively, in short (30 min) and/or long (24 hours) exposures. Results show that NP size is an important factor affecting NP percutaneous absorption. The 12 nm Fe₃O₄ NPs reached the SC and viable epidermis; 32 nm Fe₃O₄@SiO₂core/shell NPs only reached SC. However, 33 nm and 78 nm silica NPs did not permeate SC. Similar patterns were observed for NP binding affinity to SC and dermatopharmacokinetic analysis using the tape stripping method. The binding affinity determination may be a useful method to efficiently screen skin penetration of NPs.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE National Nuclear Security Administration (NNSA)

Grant/Contract Number:: AC52-07NA27344; HDTRA1-14-0005

OSTI ID:: 1603236

Report Number(s):: LLNL-JRNL-757207; 944926

Journal Information:: Toxicology Research and Application, Vol. 3, Issue na; ISSN 2397-8473

Publisher:: SageCopyright Statement

Country of Publication:: United States

Language:: English

References (34)

Analysis of Skin Permeability and Toxicological Properties of Amorphous Silica Particles Matsuo, Kazuhiko; Hirobe, Sachiko; Okada, Naoki Biological & Pharmaceutical Bulletin, Vol. 39, Issue 7 https://doi.org/10.1248/bpb.b16-00258	journal	January 2016
Estimation of the relative stratum corneum amount removed by tape stripping Jacobi, U.; Weigmann, H. -J.; Ulrich, J. Skin Research and Technology, Vol. 11, Issue 2 https://doi.org/10.1111/j.1600-0846.2005.00094.x	journal	May 2005
Binding affinity and decontamination of dermal decontamination gel to model chemical warfare agent simulants: Skin decontamination Cao, Yachao; Elmahdy, Akram; Zhu, Hanjiang Journal of Applied Toxicology, Vol. 38, Issue 5 https://doi.org/10.1002/jat.3580	journal	January 2018
Assessment of dermal toxicity of nanosilica using cultured keratinocytes, a human skin equivalent model and an in vivo model Park, Yoon-Hee; Kim, Ji Na; Jeong, Sang Hoon Toxicology, Vol. 267, Issue 1-3 https://doi.org/10.1016/j.tox.2009.10.011	journal	January 2010
Effects of Mechanical Flexion on the Penetration of Fullerene Amino Acid-Derivatized Peptide Nanoparticles through Skin Rouse, Jillian G.; Yang, Jianzhong; Ryman-Rasmussen, Jessica P. Nano Letters, Vol. 7, Issue 1 https://doi.org/10.1021/nl062464m	journal	January 2007
40nm, but not 750 or 1,500nm, Nanoparticles Enter Epidermal CD1a+ Cells after Transcutaneous Application on Human Skin Vogt, Annika; Combadiere, Behazine; Hadam, Sabrina Journal of Investigative Dermatology, Vol. 126, Issue 6 https://doi.org/10.1038/sj.jid.5700226	journal	June 2006
Penetration of Gold Nanoparticles through Human Skin: Unraveling Its Mechanisms at the Molecular Scale Gupta, Rakesh; Rai, Beena The Journal of Physical Chemistry B, Vol. 120, Issue 29 https://doi.org/10.1021/acs.jpcb.6b03212	journal	July 2016
Nanoparticles and their interactions with the dermal barrier Schneider, Marc; Stracke, Frank; Hansen, Steffi Dermato-Endocrinology, Vol. 1, Issue 4 https://doi.org/10.4161/derm.1.4.9501	journal	July 2009
Qualitative detection of single submicron and nanoparticles in human skin by scanning transmission x-ray microscopy Graf, Christina; Meinke, Martina; Gao, Qi Journal of Biomedical Optics, Vol. 14, Issue 2 https://doi.org/10.1117/1.3078811	journal	January 2009
Nanoparticles: Health Effects—Pros and Cons Gwinn, Maureen R.; Vallyathan, Val Environmental Health Perspectives, Vol. 114, Issue 12 https://doi.org/10.1289/ehp.8871	journal	December 2006
Bioavailability of Clobetasol Propionate – Quantification of Drug Concentrationsin the Stratum Corneum by Dermatopharmacokinetics UsingTape Stripping Weigmann, H. -J.; Lademann, J.; Pelchrzim, R. v. Skin Pharmacology and Physiology, Vol. 12, Issue 1-2 https://doi.org/10.1159/000029845	journal	January 1999
Quantification of the horny layer using tape stripping and microscopic techniques Lindemann, Ute; Wilken, Kathrin; Weigmann, Hans-Jürgen Journal of Biomedical Optics, Vol. 8, Issue 4 https://doi.org/10.1117/1.1609200	journal	January 2003
Nanoparticles Do Not Penetrate Human Skin—A Theoretical Perspective Watkinson, Adam C.; Bunge, Annette L.; Hadgraft, Jonathan Pharmaceutical Research, Vol. 30, Issue 8 https://doi.org/10.1007/s11095-013-1073-9	journal	May 2013
Penetration of Nanoparticles into Human Skin Liang, Xiao; Xu, Zhi; Grice, Jeffrey Current Pharmaceutical Design, Vol. 19, Issue 35 https://doi.org/10.2174/1381612811319350011	journal	September 2013
Keratin-water-NMF interaction as a three layer model in the human stratum corneum using in vivo confocal Raman microscopy Choe, ChunSik; Schleusener, Johannes; Lademann, Jürgen Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/s41598-017-16202-x	journal	November 2017
Pharmacokinetics of metallic nanoparticles: Pharmacokinetics of metallic nanoparticles Lin, Zhoumeng; Monteiro-Riviere, Nancy A.; Riviere, Jim E. Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology, Vol. 7, Issue 2 https://doi.org/10.1002/wnan.1304	journal	October 2014
Heat-separation of normal human skin for epidermal and dermal prostaglandin analysis Kassis, V.; S�ndergaard, J. Archives of Dermatological Research, Vol. 273-273, Issue 3-4 https://doi.org/10.1007/BF00409259	journal	July 1982
Variation in stratum corneum protein content as a function of anatomical site and ethnic group Raj, N.; Voegeli, R.; Rawlings, A. V. International Journal of Cosmetic Science, Vol. 38, Issue 3 https://doi.org/10.1111/ics.12274	journal	October 2015
Validation of the VapoMeter, a closed unventilated chamber system to assess transepidermal water loss vs. the open chamber TewameterR De Paepe, Kristien; Houben, Evi; Adam, Ralf Skin Research and Technology, Vol. 11, Issue 1 https://doi.org/10.1111/j.1600-0846.2005.00101.x	journal	February 2005
Functionalizing Nanoparticles with Biological Molecules: Developing Chemistries that Facilitate Nanotechnology Sapsford, Kim E.; Algar, W. Russ; Berti, Lorenzo Chemical Reviews, Vol. 113, Issue 3 https://doi.org/10.1021/cr300143v	journal	February 2013
The stratum corneum comprises three layers with distinct metal-ion barrier properties Kubo, Akiharu; Ishizaki, Itsuko; Kubo, Akiko Scientific Reports, Vol. 3, Issue 1 https://doi.org/10.1038/srep01731	journal	April 2013
Partitioning of chemicals into human stratum corneum: Implications for risk assessment following dermal exposure Surber, C. Fundamental and Applied Toxicology, Vol. 15, Issue 1 https://doi.org/10.1016/0272-0590(90)90167-I	journal	July 1990
Volume labeling with Alexa Fluor dyes and surface functionalization of highly sensitive fluorescent silica (SiO2) nanoparticles Wang, Wei; Nallathamby, Prakash D.; Foster, Carmen M. Nanoscale, Vol. 5, Issue 21 https://doi.org/10.1039/c3nr02639f	journal	January 2013
Skin Penetration and Cellular Uptake of Amorphous Silica Nanoparticles with Variable Size, Surface Functionalization, and Colloidal Stability Rancan, Fiorenza; Gao, Qi; Graf, Christina ACS Nano, Vol. 6, Issue 8 https://doi.org/10.1021/nn301622h	journal	July 2012
Penetration of nanoparticles and nanomaterials in the skin: Fiction or reality? Baroli, Biancamaria Journal of Pharmaceutical Sciences, Vol. 99, Issue 1 https://doi.org/10.1002/jps.21817	journal	January 2010
Penetration of Metallic Nanoparticles in Human Full-Thickness Skin Baroli, Biancamaria; Ennas, Maria Grazia; Loffredo, Felice Journal of Investigative Dermatology, Vol. 127, Issue 7 https://doi.org/10.1038/sj.jid.5700733	journal	July 2007
Physical and physiological effects of stratum corneum tape stripping Bashir, Saqib J.; Chew, Ai-Lean; Anigbogu, Angela Skin Research and Technology, Vol. 7, Issue 1 https://doi.org/10.1034/j.1600-0846.2001.007001040.x	journal	February 2001
An integrated view of the epidermal environmental interface Elias, Peter M.; Wakefield, Joan S. Dermatologica Sinica, Vol. 33, Issue 2 https://doi.org/10.1016/j.dsi.2015.03.008	journal	June 2015
Bioequivalence Methodologies for Topical Drug Products: In Vitro and Ex Vivo Studies with a Corticosteroid and an Anti-Fungal Drug Leal, Leila Bastos; Cordery, Sarah F.; Delgado-Charro, M. Begoña Pharmaceutical Research, Vol. 34, Issue 4 https://doi.org/10.1007/s11095-017-2099-1	journal	January 2017
Recent Progress of Nano-drug Delivery System for Liver Cancer Treatment Zhou, Feilong; Teng, Fangfang; Deng, Peizong Anti-Cancer Agents in Medicinal Chemistry, Vol. 17, Issue 14 https://doi.org/10.2174/1871520617666170713151149	journal	February 2018
Facile Synthesis of Core-shell Magnetic Mesoporous Silica Nanoparticles for pH-sensitive Anticancer Drug Delivery Shao, Dan; Wang, Zheng; Dong, Wen-fei Chemical Biology & Drug Design, Vol. 86, Issue 6 https://doi.org/10.1111/cbdd.12622	journal	August 2015
Pig Ear Skin ex Vivo as a Model for in Vivo Dermatopharmacokinetic Studies in Man Herkenne, Christophe; Naik, Aarti; Kalia, Yogeshvar N. Pharmaceutical Research, Vol. 23, Issue 8 https://doi.org/10.1007/s11095-006-9011-8	journal	July 2006
Guidance on dermal absorption Buist, Harrie; Craig, Peter; Dewhurst, Ian EFSA Journal, Vol. 15, Issue 6 https://doi.org/10.2903/j.efsa.2017.4873	journal	June 2017
Partitioning of Chemicals into Human Stratum Corneum: Implications for Risk Assessment following Dermal Exposure Surber, Christian; Wilhelm, Klaus-P; Maibach, Howard I. Toxicological Sciences, Vol. 15, Issue 1 https://doi.org/10.1093/toxsci/15.1.99	journal	January 1990

Similar Records

Effects of UVA (320-400 nm) on the barrier characteristics of the skin

Journal Article · Wed May 01 00:00:00 EDT 1991 · Journal of Investigative Dermatology; (USA) · OSTI ID:1603236

McAuliffe, D J; Blank, I H

Diffusion of (2-/sup 14/C)diazepam across hairless mouse skin and human skin

Journal Article · Fri May 01 00:00:00 EDT 1987 · J. Invest. Dermatol.; (United States) · OSTI ID:1603236

Koch, R L; Palicharla, P; Groves, M J

Diffusion of (2-/sup 14/C)diazepam across isolated hairless mouse stratum corneum/epidermal tissues

Journal Article · Tue Mar 01 00:00:00 EST 1988 · J. Invest. Dermatol.; (United States) · OSTI ID:1603236

Koch, R L; Palicharla, P; Groves, M J

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Iron oxide and silica oxide nanoparticles
in vitro human skin absorption
stratum corneum binding
dermatopharmacokinetic
tape stripping
nanoparticle size-related penetration

Title: Effect of iron and silica nanoparticles’ size on in vitro human skin binding and penetration

Citation Formats

References (34)

Similar Records

Related Subjects