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Title: Overexpression of a Prefoldin β subunit gene reduces biomass recalcitrance in the bioenergy crop Populus

Journal Article · · Plant Biotechnology Journal
DOI:https://doi.org/10.1111/pbi.13254· OSTI ID:1566924
ORCiD logo [1];  [2];  [3];  [4];  [1]; ORCiD logo [5];  [6];  [6];  [6];  [7];  [7];  [1];  [1];  [1];  [1];  [7];  [1];  [8];  [1];  [1] more »; ORCiD logo [1] « less
  1. Biosciences Division Oak Ridge National Laboratory Oak Ridge TN USA, Center for Bioenergy Innovation Oak Ridge National Laboratory Oak Ridge TN USA
  2. Biosciences Division Oak Ridge National Laboratory Oak Ridge TN USA, Center for Bioenergy Innovation Oak Ridge National Laboratory Oak Ridge TN USA, Department of Plant Sciences University of Tennessee Knoxville TN USA
  3. Chemical &, Biomolecular Engineering University of Tennessee Knoxville TN USA
  4. Chemical &, Biomolecular Engineering University of Tennessee Knoxville TN USA, College of Textiles Donghua University Shanghai China
  5. Biosciences Division Oak Ridge National Laboratory Oak Ridge TN USA
  6. ArborGen Inc. Ridgeville SC USA
  7. U.S. Department of Energy Joint Genome Institute Walnut Creek CA USA
  8. U.S. Department of Energy Joint Genome Institute Walnut Creek CA USA, HudsonAlpha Institute for Biotechnology Huntsville AL USA
+ Show Author Affiliations

Prefoldin (PFD) is a group II chaperonin that is ubiquitously present in the eukaryotic kingdom. Six subunits (PFD1-6) form a jellyfish-like heterohexameric PFD complex and function in protein folding and cytoskeleton organization. However, little is known about its function in plant cell wall-related processes. Here, we report the functional characterization of a PFD gene from em>Populus deltoides, designated as PdPFD2.2 There are two copies of PFD2 in em>Populus and PdPFD2.2 was ubiquitously expressed with high transcript abundance in the cambial region. PdPFD2.2 can physically interact with DELLA protein RGA1_8g, and its subcellular localization is affected by the interaction. In P. deltoides transgenic plants overexpressing PdPFD2.2, the lignin syringyl/guaiacyl ratio was increased, but cellulose content and crystallinity index were unchanged. In addition, the total released sugar (glucose and xylose) amounts were increased by 7.6% and 6.1%, respectively, in two transgenic lines. Transcriptomic and metabolomic analyses revealed that secondary metabolic pathways, including lignin and flavonoid biosynthesis, were affected by overexpressing PdPFD2.2. A total of eight hub transcription factors (TFs) were identified based on TF binding sites of differentially expressed genes in Populus transgenic plants overexpressing PdPFD2.2. In addition, several known cell wall-related TFs, such as MYB3, MYB4, MYB7, TT8, and XND1, were affected by overexpression of PdPFD2.2. These results suggest that overexpression of PdPFD2.2 can reduce biomass recalcitrance and PdPFD2.2 is a promising target for genetic engineering to improve feedstock characteristics to enhance biofuel conversion and reduce the cost of lignocellulosic biofuel production.

Research Organization:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
Grant/Contract Number:
AC02‐05CH11231; AC05‐00OR22725; AC05-00OR22725; AC02‐05CH1123; AC02-05CH11231
OSTI ID:
1566924
Alternate ID(s):
OSTI ID: 1561676; OSTI ID: 1566925; OSTI ID: 1616121
Journal Information:
Plant Biotechnology Journal, Journal Name: Plant Biotechnology Journal; ISSN 1467-7644
Publisher:
Society for Experimental Biology; Association of Applied BiologyCopyright Statement
Country of Publication:
United Kingdom
Language:
English
Citation Metrics:
Cited by: 14 works
Citation information provided by
Web of Science

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Figures / Tables (5)

Figure 1(p. 4)figure Figure 1
Figure 2(p. 6)figure Figure 2
Figure 3(p. 7)figure Figure 3
Figure 4(p. 8)figure Figure 4
Figure 5(p. 9)figure Figure 5

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Related Subjects

09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
Populus
prefoldin
biofuels
cell wall recalcitrance
lignin
S/G ratio
transcriptome
metabolome
cell wall reclcitrance