High-resolution crystal structure of Streptococcus agalactiae glyceraldehyde-3-phosphate dehydrogenase

Journal Article · Fri Mar 23 00:00:00 EDT 2018 · Acta Crystallographica. Section F, Structural Biology Communications

DOI:https://doi.org/10.1107/S2053230X18003801· OSTI ID:1502235

Zhou, Kang; Fan, Xiaojiao; Li, Yuelong; Zhang, Caiying; Jin, Tengchuan

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional enzyme that plays critical roles in bacterial pathogenesis in some pathogenic bacteria. In this study, the crystal structure of group B streptococcus GAPDH was determined at 1.36 Å resolution. The structure contained an asymmetric mixed holo tetramer, with two NAD ligands bound to two protomers. Further structural analysis identified interesting phosphate ion-binding sites, which shed light on its catalytic mechanism.

• Research Organization: Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
• Sponsoring Organization: FOREIGN
• OSTI ID: 1502235
• Journal Information: Acta Crystallographica. Section F, Structural Biology Communications, Vol. 74, Issue 4; ISSN 2053-230X
• Publisher: International Union of Crystallography
• Country of Publication: United States
• Language: ENGLISH