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Title: Rapid Nucleic Acid Extraction and Purification Using a Miniature Ultrasonic Technique

Journal Article · · Micromachines
DOI:https://doi.org/10.3390/mi8070228· OSTI ID:1399498
 [1];  [2];  [3];  [4];  [5];  [1]
  1. Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). Nano and Micro Sensors Dept.
  2. Imagion Biosystems, Inc., Albuquerque, NM (United States)
  3. Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). MEMS Technologies Dept.
  4. Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). Bioenergy and Defense Technologies Dept.
  5. Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). Physics Based Microsystems Dept.
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Miniature ultrasonic lysis for biological sample preparation is a promising technique for efficient and rapid extraction of nucleic acids and proteins from a wide variety of biological sources. Acoustic methods achieve rapid, unbiased, and efficacious disruption of cellular membranes while avoiding the use of harsh chemicals and enzymes, which interfere with detection assays. In this work, a miniature acoustic nucleic acid extraction system is presented. Using a miniature bulk acoustic wave (BAW) transducer array based on 36° Y-cut lithium niobate, acoustic waves were coupled into disposable laminate-based microfluidic cartridges. To verify the lysing effectiveness, the amount of liberated ATP and the cell viability were measured and compared to untreated samples. The relationship between input power, energy dose, flow-rate, and lysing efficiency were determined. DNA was purified on-chip using three approaches implemented in the cartridges: a silica-based sol-gel silica-bead filled microchannel, nucleic acid binding magnetic beads, and Nafion-coated electrodes. Using E. coli, the lysing dose defined as ATP released per joule was 2.2× greater, releasing 6.1× more ATP for the miniature BAW array compared to a bench-top acoustic lysis system. An electric field-based nucleic acid purification approach using Nafion films yielded an extraction efficiency of 69.2% in 10 min for 50 µL samples.

Research Organization:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Organization:
USDOE National Nuclear Security Administration (NNSA)
Grant/Contract Number:
NA0003525
OSTI ID:
1399498
Report Number(s):
SAND-2017-3875J; 652447
Journal Information:
Micromachines, Vol. 8, Issue 7; ISSN 2072-666X
Publisher:
MDPICopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 4 works
Citation information provided by
Web of Science

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Cited By (2)

Smartphone operable centrifugal system (SOCS) for on-site DNA extraction from foodborne bacterial pathogen journal May 2019
Application of Microfluidic Methodology for the Analysis of DNA journal January 2018

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59 BASIC BIOLOGICAL SCIENCES
77 NANOSCIENCE AND NANOTECHNOLOGY
acoustic lysis
microfluidic
nucleic acids
extraction
purification
separation
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