Two mechanisms for dissipation of excess light in monomeric and trimeric light-harvesting complexes

Dall'Osto, Luca; Cazzaniga, Stefano; Bressan, Mauro; Paleček, David; Židek, Karel; Niyogi, Krishna K.; Fleming, Graham R.; Zigmantas, Donatas; Bassi, Roberto

doi:10.1038/nplants.2017.33

Title: Two mechanisms for dissipation of excess light in monomeric and trimeric light-harvesting complexes

Journal Article · Mon Apr 10 00:00:00 EDT 2017 · Nature Plants

DOI:https://doi.org/10.1038/nplants.2017.33· OSTI ID:1393220

^[1]; Cazzaniga, Stefano ^[1]; Bressan, Mauro ^[1]; Paleček, David ^[2]; Židek, Karel ^[2]; Niyogi, Krishna K. ^[3]; Fleming, Graham R. ^[4]; Zigmantas, Donatas ^[2];

^[5]

Univ. di Verona, Verona (Italy). Dipartimento di Biotecnologie
Lund Univ. (Sweden). Dept. of Chemical Physics
Univ. of California, Berkeley, CA (United States). Howard Hughes Medical Inst., Dept. of Plant and Microbial Biology; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Molecular Biophysics and Integrated Bioimaging Division
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Molecular Biophysics and Integrated Bioimaging Division; Univ. of California, Berkeley, CA (United States). Dept. of Chemistry, Graduate Group in Applied Science and Technology
Univ. di Verona, Verona (Italy). Dipartimento di Biotecnologie; Consiglio Nazionale delle Ricerche (CNR), Firenze (Italy). Istituto per la Protezione delle Piante (IPP)

Oxygenic photoautotrophs require mechanisms for rapidly matching the level of chlorophyll excited states from light harvesting with the rate of electron transport from water to carbon dioxide. These photoprotective reactions prevent formation of reactive excited states and photoinhibition. The fastest response to excess illumination is the so-called non-photochemical quenching which, in higher plants, requires the luminal pH sensor PsbS and other yet unidentified components of the photosystem II antenna. Both trimeric light-harvesting complex II (LHCII) and monomeric LHC proteins have been indicated as site(s) of the heat-dissipative reactions. Different mechanisms have been proposed: Energy transfer to a lutein quencher in trimers, formation of a zeaxanthin radical cation in monomers. Here, we report on the construction of a mutant lacking all monomeric LHC proteins but retaining LHCII trimers. Its non-photochemical quenching induction rate was substantially slower with respect to the wild type. A carotenoid radical cation signal was detected in the wild type, although it was lost in the mutant. Here, we conclude that non-photochemical quenching is catalysed by two independent mechanisms, with the fastest activated response catalysed within monomeric LHC proteins depending on both zeaxanthin and lutein and on the formation of a radical cation. Trimeric LHCII was responsible for the slowly activated quenching component whereas inclusion in supercomplexes was not required. Finally, this latter activity does not depend on lutein nor on charge transfer events, whereas zeaxanthin was essential.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

DOE Contract Number:: AC02-05CH11231

OSTI ID:: 1393220

Journal Information:: Nature Plants, Vol. 3, Issue 5; ISSN 2055-0278

Publisher:: Nature Publishing Group

Country of Publication:: United States

Language:: English

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