Apparent lack of restriction fragment length polymorphisms in chromosome specific arrays of gamma satellite DNA
- Univ. of Alberta, Edmonton (Canada)
The centromeric regions of human chromosomes are known to be composed primarily of repeated satellite DNA sequences which are tandemly organized into large arrays. A new class of centromeric satellite DNA, designated as human gamma satellite DNA, was originally identified in the centromeric region of human chromosome 8 (gamma 8) and found to be characterized by monomeric units of 220 bp. More recently, a cosmid clone was localized by fluorescence in situ hybridization to the centromeric region of the human X chromosome and was found to lack alpha satellite DNA. A 1205 bp EcoRI subclone (2D12/E2) of this X-centromere specific cosmid clone revealed approximately 5 monomers of 220 bp which had approximately 62% DNA sequence similarly to the gamma 8 monomers. Genomic DNA from ten unrelated individuals (6 males & 4 females) were digested in agarose plugs with Sstl, separated by pulsed field gel electrophoresis and probed with {sup 32}P-labeled 2D12/E2. A high molecular weight fragment of {approximately}34 kb was detected in all individuals. A similar experiment using Hpal revealed high molecular weight fragments of {approximately} 54 kb and {approximately}70 kb in all individuals. When the Southern blots were stripped and reprobed with the 704 bp, gamma-8 satellite DNA probe (50E1), Sstl fragments of {approximately} 34 kb were seen in all individuals tested. This apparent lack of polymorphisms in chromosome specific arrays of gamma satellite DNA among unrelated individuals is contrasting to the high frequency of polymorphisms seen in arrays of alpha satellite DNA. This may indicate a physical or functional constraint against sequence variation in gamma satellite DNA arrays.
- OSTI ID:
- 133711
- Report Number(s):
- CONF-941009-; ISSN 0002-9297; TRN: 95:005313-0442
- Journal Information:
- American Journal of Human Genetics, Vol. 55, Issue Suppl.3; Conference: 44. annual meeting of the American Society of Human Genetics, Montreal (Canada), 18-22 Oct 1994; Other Information: PBD: Sep 1994
- Country of Publication:
- United States
- Language:
- English
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