Saccharification and fermentation of dilute pretreated corn fiber to ethanol using a recombinant xylose-fermenting saccharomyces
- Purdue Univ., West Lafayette, IN (United States)
A recombinant yeast 1400 (pLNH33), that can ferment both glucose and xylose effectively was evaluated for simultaneous saccarification and fermentation (SSF) of dilute acid pretreated corn fiber. LNH33 is able to utilize both glucose and xylose to produce ethanol simultaneously in this SSF process. An ethanol concentration of 40 g/l was obtained in both batch and fed-batch SSF. The cellulose in this process performed a key role that not only hydrolyzed cellulose to glucose but also fluidized the acid-treated corn fiber slurry. The fluidized of corn fiber slurry is an important step that rendered the high-solid fermentation possible. Supplementation of erogenous glucose to the fermentation broth after 68 hours of SSF raised the ethanol concentration to about 100 g/l indicating that LNH33 has retained its ethanol tolerance characteristic of its parent. The rate of ethanol production is limited by the slower rate of xylose utilization which is about one-fifth of glucose utilization.
- OSTI ID:
- 126128
- Report Number(s):
- CONF-950402-; TRN: 95:006086-0101
- Resource Relation:
- Conference: 209. American Chemical Society (ACS) national meeting, Anaheim, CA (United States), 2-6 Apr 1995; Other Information: PBD: 1995; Related Information: Is Part Of 209th ACS national meeting; PB: 2088 p.
- Country of Publication:
- United States
- Language:
- English
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