Bacterial Pleckstrin Homology Domains: A Prokaryotic Origin for the PH Domain

Xu, Qingping; Bateman, Alex; Finn, Robert D.; Abdubek, Polat; Astakhova, Tamara; Axelrod, Herbert L.; Bakolitsa, Constantina; Carlton, Dennis; Chen, Connie; Chiu, Hsiu-Ju; Chiu, Michelle; Clayton, Thomas; Das, Debanu; Deller, Marc C.; Duan, Lian; Ellrott, Kyle; Ernst, Dustin; Farr, Carol L.; Feuerhelm, Julie; Grant, Joanna C.; Grzechnik, Anna; Han, Gye Won; Jaroszewski, Lukasz; Jin, Kevin K.; Klock, Heath E.; Knuth, Mark W.; Kozbial, Piotr; Krishna, S. Sri; Kumar, Abhinav; Marciano, David; McMullan, Daniel; Miller, Mitchell D.; Morse, Andrew T.; Nigoghossian, Edward; Nopakun, Amanda; Okach, Linda; Puckett, Christina; Reyes, Ron; Rife, Christopher L.; Sefcovic, Natasha; Tien, Henry J.; Trame, Christine B.; van den Bedem, Henry; Weekes, Dana; Wooten, Tiffany; Hodgson, Keith O.; Wooley, John; Elsliger, Marc-André; Deacon, Ashley M.; Godzik, Adam; Lesley, Scott A.; Wilson, Ian A.

doi:10.1016/j.jmb.2009.11.006

Title: Bacterial Pleckstrin Homology Domains: A Prokaryotic Origin for the PH Domain

Journal Article · Mon Feb 01 00:00:00 EST 2010 · Journal of Molecular Biology

DOI:https://doi.org/10.1016/j.jmb.2009.11.006· OSTI ID:1209400

Xu, Qingping; Bateman, Alex; Finn, Robert D.; Abdubek, Polat; Astakhova, Tamara; Axelrod, Herbert L.; Bakolitsa, Constantina; Carlton, Dennis; Chen, Connie; Chiu, Hsiu-Ju; Chiu, Michelle; Clayton, Thomas; Das, Debanu; Deller, Marc C.; Duan, Lian; Ellrott, Kyle; Ernst, Dustin; Farr, Carol L.; Feuerhelm, Julie; Grant, Joanna C. more »

Pleckstrin homology (PH) domains have been identified only in eukaryoticproteins to date. We have determined crystal structures for three membersof an uncharacterized protein family (Pfam PF08000), which providecompelling evidence for the existence of PH-like domains in bacteria (PHb).The first two structures contain a single PHbdomain that forms a dome-shaped, oligomeric ring with C5symmetry. The third structure has anadditional helical hairpin attached at the C-terminus and forms a similar butmuch larger ring with C12symmetry. Thus, both molecular assembliesexhibit rare, higher-order, cyclic symmetry but preserve a similararrangement of their PHbdomains, which gives rise to a conservedhydrophilic surface at the intersection of theβ-strands of adjacentprotomers that likely mediates protein–protein interactions. As a result ofthese structures, additional families of PHbdomains were identified,suggesting that PH domains are much more widespread than originallyanticipated. Thus, rather than being a eukaryotic innovation, the PHdomain superfamily appears to have existed before prokaryotes andeukaryotes diverged.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: AC03-76SF00098

OSTI ID:: 1209400

Alternate ID(s):: OSTI ID: 1623694

Journal Information:: Journal of Molecular Biology, Vol. 396, Issue 1; ISSN 0022-2836

Publisher:: ElsevierCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 25 works

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References (58)

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