Method for metabolizing carbazole in petroleum
Patent
·
OSTI ID:1175494
A method for selective cleavage of C--N bonds genes that encode for at least one enzyme suitable for conversion of carbazole to 2-aminobiphenyl-2,3-diol are combined with a gene encoding an amidase suitable for selectively cleaving a C--N bond in 2-aminobiphenyl-2,3-diol, forming an operon that encodes for cleavage of both C--N bonds of said carbazole. The operon is inserted into a host culture which, in turn, is contacted with the carbazole, resulting in selective cleavage of both C--N bonds of the carbazole. Also disclosed is a new microorganism that expresses a carbazole degradation trait constitutively and a method for degrading carbazole employing this microorganism.
- Research Organization:
- National Energy Technology Laboratory (NETL), Pittsburgh, PA, Morgantown, WV (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC26-99BC15219
- Assignee:
- Alps Electric Co., Ltd. (Tokyo, JP)
- Patent Number(s):
- 6,943,006
- Application Number:
- 10/658,691
- OSTI ID:
- 1175494
- Country of Publication:
- United States
- Language:
- English
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