Capillary Isotachophoresis-Nanoelectrospray Ionization-Selected Reaction Monitoring MS via a Novel Sheathless Interface for High Sensitivity Sample Quantification

Journal Article · Fri Jun 21 00:00:00 EDT 2013 · Analytical Chemistry, 85(15):7308-7315

DOI:https://doi.org/10.1021/ac401202c· OSTI ID:1091482

Wang, Chenchen ^[1]; Lee, Cheng S.; Smith, Richard D.; Tang, Keqi

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1. Chloe

A novel sheathless CITP/CZE-MS interface featuring a large i.d. separation capillary and a detachable small i.d. ESI emitter was developed in this study to simultaneously achieve large sample loading capacity and stable nanoESI operation. Crucial operating parameters, including sample loading volume, flow rate, and separation window, were systematically investigated to attain optimum CITP/CZE separation efficiency and MS detection sensitivity. The performance of CITP/CZE-nanoESI-MS using the new sheathless interface was evaluated for its achievable low limit of quantitation (LOQ) by analyzing targeted peptides, leu-enkephalin and angiotensin II, spiked in a BSA tryptic digest matrix at different concentrations. A linear dynamic range spanning 4.5 orders of magnitude and a 10 pM LOQ with excellent measurement reproducibility were obtained experimentally for both targeted peptides, representing a 5-fold sensitivity improvement as compared to using the sheath liquid interface developed previously.

• Research Organization: Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 1091482
• Report Number(s): PNNL-SA-95213; 47301; 400412000
• Journal Information: Analytical Chemistry, 85(15):7308-7315, Journal Name: Analytical Chemistry, 85(15):7308-7315
• Country of Publication: United States
• Language: English

Related Subjects

Environmental Molecular Sciences Laboratory