Identifying Low pH Active and Lactate-Utilizing Taxa within Oral Microbiome Communities from Healthy Children Using Stable Isotope Probing Techniques
Many human microbial infectious diseases including dental caries are polymicrobial in nature and how these complex multi-species communities evolve from a healthy to a diseased state is not well understood. Although many health- or disease-associated oral microbes have been characterized in vitro, their physiology in vivo in the presence of the complex oral microbiome is difficult to determine with current approaches. In addition, about half of these oral species remain uncultivated to date and little is known except their 16S rRNA sequence. Lacking culture-based physiological analyses, the functional roles of uncultivated microorganisms will remain enigmatic despite their apparent disease correlation. To start addressing these knowledge gaps, we applied a novel combination of in vivo Magnetic Resonance Spectroscopy (MRS) with RNA and DNA based Stable Isotope Probing (SIP) to oral plaque communities from healthy children for temporal monitoring of carbohydrate utilization, organic acid production and identification of metabolically active and inactive bacterial species.
- Research Organization:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 1036913
- Report Number(s):
- PNNL-SA-83915; 25694; TRN: US201206%%660
- Journal Information:
- PLoS One, 7(3):Article No. e32219, Vol. 7, Issue 3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
60 APPLIED LIFE SCIENCES
CARBOHYDRATES
CARIES
CHILDREN
COMMUNITIES
DISEASES
DNA
FUNCTIONALS
IN VITRO
IN VIVO
INFECTIOUS DISEASES
MAGNETIC RESONANCE
MICROORGANISMS
MONITORING
ORGANIC ACIDS
PHYSIOLOGY
PRODUCTION
RNA
SPECTROSCOPY
STABLE ISOTOPES
oral communities
polymicrobial
microbiome
Environmental Molecular Sciences Laboratory