FISH 'N' Chips : a single cell genomic analyzer for the human microbiome.
- Joint Genome Institute, Walnut Creek, CA
- New York University, New York, NY
Uncultivable microorganisms likely play significant roles in the ecology within the human body, with subtle but important implications for human health. Focusing on the oral microbiome, we are developing a processor for targeted isolation of individual microbial cells, facilitating whole-genome analysis without the need for isolation of pure cultures. The processor consists of three microfluidic modules: identification based on 16S rRNA fluorescence in situ hybridization (FISH), fluorescence-based sorting, and encapsulation of individual selected cells into small droplets for whole genome amplification. We present here a technique for performing microscale FISH and flow cytometry, as a prelude to single cell sorting.
- Research Organization:
- Sandia National Laboratories (SNL), Albuquerque, NM, and Livermore, CA (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC04-94AL85000
- OSTI ID:
- 1030341
- Report Number(s):
- SAND2010-6714C; TRN: US201124%%129
- Resource Relation:
- Conference: Proposed for presentation at the MicroTAS 2010 Conference held October 3-7, 2010 in Groningen, The Netherlands.
- Country of Publication:
- United States
- Language:
- English
Similar Records
The “Most Wanted” Taxa from the Human Microbiome for Whole Genome Sequencing
Nearly finished genomes produced using gel microdroplet culturing reveal substantial intraspecies genomic diversity within the human microbiome