Brominated Tyrosine and Polyelectrolyte Multilayer Analysis by Laser Desorption VUV Postionization and Secondary Ion Mass Spectrometry

Journal Article · Mon Mar 14 00:00:00 EDT 2011 · Analytical Chemistry

OSTI ID:1012374

Blaze, Melvin M. T.; Takahashi, Lynelle; Zhou, Jia; Ahmed, Musahid; Gasper, Gerald; Pleticha, F Douglas; Hanley, Luke

The small molecular analyte 3,5-dibromotyrosine (Br2Y) and chitosan-alginate polyelectrolyte multilayers (PEM) with and without adsorbed Br2Y were analyzed by laser desorption postionization mass spectrometry (LDPI-MS). LDPI-MS using 7.87 eV laser and tunable 8 ? 12.5 eV synchrotron vacuum ultraviolet (VUV) radiation found that desorption of clusters from Br2Y films allowed detection by≤8 eV single photon ionization. Thermal desorption and electronic structure calculations determined the ionization energy of Br2Y to be ~;;8.3?0.1 eV and further indicated that the lower ionization energies of clusters permitted their detection at≤8 eV photon energies. However, single photon ionization could only detect Br2Y adsorbed within PEMs when using either higher photon energies or matrix addition to the sample. All samples were also analyzed by 25 keV Bi3 + secondary ion mass spectrometry (SIMS), with the negative ion spectra showing strong parent ion signal which complemented that observed by LDPI-MS. The negative ion SIMS depended strongly on the high electron affinity of this specific analyte and the analyte?s condensed phase environment.

• Research Organization: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
• Sponsoring Organization: Chemical Sciences Division
• DOE Contract Number: DE-AC02-05CH11231
• OSTI ID: 1012374
• Report Number(s): LBNL-4436E; TRN: US1102250
• Journal Information: Analytical Chemistry, Journal Name: Analytical Chemistry
• Country of Publication: United States
• Language: English

Related Subjects

99 GENERAL AND MISCELLANEOUS
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
AFFINITY
ANIONS
DESORPTION
DETECTION
ELECTRONIC STRUCTURE
IONIZATION
LASERS
MASS SPECTROSCOPY
PHOTONS
RADIATIONS
SPECTRA
SYNCHROTRONS
TYROSINE
Brominated
Tyrosine
Polyelectrolyte
Multilayer
Desorption
VUV
Postionization
Secondary
Mass Spectrometry